Activation of hamster mast cells for IgE — Mediated histamine release |
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Authors: | Janina Wyczo?kowska Annie Prouvost-Danon Czes?aw Ma?liński |
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Institution: | (1) Department of Biogenic Amines of Polish Academy of Sciences, Narutowicza 60, 90-136 ód , Poland;(2) CNRS, G.R. 31, Immunopharmacologie Expérimentale, Institut des Cordeliers, Paris, France |
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Abstract: | The conditions for active sensitization of hamster peritoneal and pleural mast cells and IgE-induced histamine release as well as cell desensitization were defined. Immunization of hamsters with ovalbumin (5 g) in Al/OH/3 gel (5 mg) with several boosters resulted in sensitization of peritoneal and pleural mast cells; in the presence of extracellular Ca++, pH of medium 7.2 and at 37°C these cells released up to 70% of histamine on the challenge with specific antigen. Partial release was observed when the cells were challenged with antigen in the absence of extracellular calcium. The rate of release is high during the first seconds of activation and is complete at 1 min. 30 min preincubation of peritoneal and pleural mast cells in calcium-free conditions (in the presence of 4 mM EDTA) resulted in complete desensitization of cells to subsequent action of antigen in potimal conditions. The present experiments demonstrate, that hamster peritoneal and pleural mast cells can be a useful model system forin vitro studies of the mechanisms of IgE-induced cell activation. |
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