圆斑酶联免疫吸附试验诊断囊虫病——生物素-亲和素酶复合物在该方法中的应用

目的：应用生物素－亲和素－酶复合物系统，建立一种繁感、特异、经济简便的囊虫病血清学诊断。方法：生物素－亲和素－酶复合物圆斑酶联免疫吸附试验（ＡＢＣ－Ｄｏｔ－ＥＬＩＳＡ）、滤纸血斑ＡＢＣ－Ｄｏｔ－ＥＬＩＳＡ。结果：ＡＢＣ－Ｄｏｔ－ＥＬＩＳＡ 诊断囊虫病，检出率（９５．９２％ ）远较圆斑酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）高８３．６７％ ，平均几何滴度（１∶２ ８０１．７３）约为Ｄｏｔ－ＥＬＩＳＡ（１∶５８７．２６）的５ 倍；用该方法对４℃条件下保存３ 月的患者滤纸血斑样本进行检测，阳性率为９４．７４％ （５４／５７），无假阳性反应（０／４５）；对相同患者血清和滤纸血斑双份血样进行检测，阳性率均为９０％ （２０／２２）。结论：该方法提高了检出率，简化了采样及送样程序，适合于边远地区和用于流行病学调查。

DIAGNOSIS OF CYSTICERCOSIS BY DOT ENZYME LINKED IMMUNOSORBANT ASSAY──AN APPLICATION OF BIOTIN-AVIDIN-ENZYME COMPLEX IN DOT-ELISA

Objective: To establish a highly sensitive, specific and simple approach for serodiagnosis of cysticercosis Method: ABC Dot ELISA, Dot ELISA, and blood samples taken on filter paper ABC Dot ELISA Results: Cysticercosis was detected by ABC Dot ELISA and Dot ELISA, the ratio of positive reaction of the former(95 92%) being higher than that of the latter(83 67%) The geometric mean titer of ABC Dot ELISA(1∶2 801 73) was about 5 times of Dot ELISA(1∶587 26) 57 blood samples taken on filter paper were detected by ABC Dot ELISA and 54 of which were positive(94 74%) No false positive reaction was noticed in the same samples of the healthy persons in the control group(0/45) Serum and blood samples on filter paper taken from same individuals were assayed by the ABC Dot ELISA with the same results Conclusion: It is a high sensitive, specific, safe and simple method for the serodiagnosis of cysticercosis in remote areas as well as for epidemiological study