卵巢早衰和不孕症患者血清中抗透明带抗体 及Th1/Th2细胞因子的测定及意义

目的:探讨抗透明带抗体的表达和Th1/Th2细胞因子的改变在卵巢早衰(POF)和不孕症患者发病中的作用。方法:用ELISA法检测21例POF、20例不明原因不孕症和20例健康女性的血清中抗透明带抗体的表达、IFN-γ、IL-4和IL-10水平。结果:POF组和不孕组血清中抗透明带抗体的阳性率均明显高于对照组(P<0.01)。POF组和不孕组血清中IFN-γ/IL-10比值均明显高于对照组(P<0.01),不孕组IFN-γ/IL-4比值明显高于对照组(P<0.01)。结论:抗透明带抗体的表达和Th1/Th2细胞因子比值增高在POF及不孕症妇女的发病过程中起到重要的作用。

The detection and significance of anti-zona pellucida antibody and Th1/Th2 cytokines in serum in patients with premature ovarian failure and infertility

Objective:To explore the effects of anti-zona pellucida antibody expression and Th1/Th2 cytokines change on onset of premature ovarian failure(POF) and infertility.Methods:The expression anti-zona pellucida antibody and the levels of IFN-γ,IL-4 and IL-10 from 21 women with POF,20 women with unexplained infertility and 20 healthy women were detected by enzyme-linked immunosorbent assay.Results:The positive rates of anti-zona pellucida antibody in POF women and infertile women were significantly higher than that in healthy women(P<0.01).The ratios of IFN-γ/IL-10 in POF women and infertile women were significantly higher than that in healthy women(P<0.01),the ratio of IFN-γ/IL-4 in infertile women was significantly higher than that in healthy women(P<0.01).Conclusion:The expression of anti-zona pellucida antibody and the high ratio of Th1/Th2 play an important role in the onset of POF and infertility.