显微镜观察药物敏感性技术检测结核分枝杆菌应用效果观察

目的 探讨显微镜观察药物敏感性检测（MODS）方法用于结核分枝杆菌（MTB）分离培养和耐药检测的应用效果.方法 收集276份肺结核临床疑似患者的痰标本,用MGIT 960 TB、罗氏法、MODS法检测MTB.用MODS法对80株MTB临床分离株进行药敏检测,并将结果与传统罗氏法进行比较.结果 MODS技术直接检测痰标本的阳性率为46％,阳性检出时间中位数为9 d（7～12 d）.MODS检测链霉素、异烟肼、利福平、乙胺丁醇药敏结果与传统罗氏法符合率分别为95.0％、96.25％、97.5％、85.0％,检测敏感度分别为83.3％、95.65％、96.15％、70.83％,特异度分别为98.3％、96.50％、98.15％、91.07％.结论 MODS方法是一种快速简便、费用低、准确的诊断和药敏检测方法,具有广泛的应用前景.

Application of microscopic observation drug susceptibility assay in detection of mycobacterium tuberculosis

Objective To investigate the application effect of microscopic observation drug susceptibility （MODS） assay on mycobacterium tuberculosis （MTB） culture and detection of drug-resistance.Methods MTB in 276 consecutive sputum samples taken from suspected patients with tuberculosis was tested by using MGIT 960 TB,L-J culture and MODS assay,and then we used MODS assay to detect drug susceptibility of MTB in 80 strains of clinical isolates and compared the results with that of the L-J.Results The detection rate of 276 sputum samples collected from TB suspects by MODS assay was 46%,and the median was 9 days （7-12 d）.The coincidence rates of the susceptibility to streptomycin,isoniazid,rifampicin and ethambutol detected by MODS and L-J were 95.0%,96.25%,97.5% and 85.0%,respectively; the sensitivities of MODS method were 83.3%,95.65,96.15% and 70.83%,and the specificities were 98.3%,96.50%,98.15% and 91.07%,respectively.Condusion MODS assay is a simple,low-cost,accurate diagnosis and drug sensitivity test method,which is appropriate for diagnosis of TB.