抑制人喉癌细胞生长的p53基因反转录病毒重组体的构建

野生型ｐ５３基因在细胞增殖与分化的调控过程中起着重要作用。已有研究表明，人喉癌中存在ｐ５３基因的结构异常或表达异常。本实验根据这一特点，设计出能整合在人喉癌细胞染色体上并适宜表达的野生型ｐ５３基因反转录病毒重组体Ｐｃ５３Ｎ２Ａ。通过体外实验证明，这一重组体能够以基因替代的方式，恢复ｐ５３基因的功能，抑制人喉癌细胞的生长。

Construction of the wt-p53 Gene Recombinant Retroviral Vector Inhibiting the Growth of Laryngocarcinoma Cell Line

We have constructed wt-p53 gene recombinant retroviral vector.The p53 cDNA with positive regulative sequence was subcloned into the Xho I site between the two LTRs of the N2A retroviral vector which contained the cytomegalovirus promotor/enhancer for expression and a neomycin resistance gene allowing G418 selection in reverse orientation and established the PA317 packaging cell line producing virus.The recipient cell line of Hep2(laryngocarcinoma) containing the abnormal p53 gene was transfected in vitro with fresh retroviral stock produced.The result showed that the contructed wt-p53 gene reconlbinant retroviral vector was able to suppress growth of laryngocarcinoma cell in vitro. These observations indicate that recombinant retrovirous expressing wild-type p5 3 may be the useful vectors for gene therapy of human laryngocarcinoma.