急性白血病患者bcl-2,c-myc基因的表达及两者之间的关系

目的 :探讨急性白血病 (AL)患者bcl 2和c myc基因的表达和两者之间的关系。方法 :原位分子杂交法检测bcl 2和c mycmRNA ;细胞免疫化学方法检测bcl 2 ,c myc蛋白表达。结果 :AL患者bcl 2 ,c mycmRNA和蛋白质均明显增加 ;急性淋巴细胞白血病与急性非淋巴细胞白血病bcl 2 ,c mycmRNA和蛋白质无明显差别 ;bcl 2mRNA与蛋白质 (r =0 .784,P <0 .0 1) ,c mycmRNA与蛋白质 (r =0 .887,P <0 .0 1) ;bcl 2mRNA与c mycmRNA (r =0 .888,P <0 .0 1) ,以及bcl 2蛋白与c myc蛋白 (r=0 .735 ,P <0 .0 1)均呈直线正相关。结论 :bcl 2 ,c myc基因在急性白血病的发生发展中起着重要作用。

Expression of bcl-2, c-myc genes and their relationship in patients with acute leukemia

Objective：This paper was to explore the expressions ofbcl-2，c-myc genes and the relationship between them in bone marrow from patients with acute leukemia (AL). Methods：In situ hybridization was used to detect bcl-2 and c-myc mRNA. Immune cytochemistry was used to detect bcl-2 and c-myc protein. Resuls：The expressions of bcl-2, c-myc mRNA and proteins were significantly increased in AL; There were not significant differences in bcl-2 mRNA and protein, c-myc mRNA and protein between the patients with acute lymphocytic leukemia and acute nonlymphocytic leukemia; There were linear correlations between bcl-2 mRNA and bcl-2 protein, between c-myc mRNA and c-myc protein, between bcl-2 mRNA and c-myc mRNA, between bcl-2 protein and c-myc protein. Conclusion：bcl-2 and c-myc genes play an important role in pathogenesis and the development of AL.