| 黄芪皂苷的提取及含量测定 |
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| 引用本文： | 王培培,许杜娟,夏泉. 黄芪皂苷的提取及含量测定[J]. 中国实验方剂学杂志, 2010, 16(4): 27-30 |
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| 作者姓名： | 王培培 许杜娟 夏泉 |
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| 作者单位： | 1. 安徽医科大学药学院,合肥,230032;安徽医科大学第一附属医院药剂科国家中医药管理局中药化学三级实验室,合肥,230022
2. 安徽医科大学第一附属医院药剂科国家中医药管理局中药化学三级实验室,合肥,230022 |
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| 基金项目： | 安徽省自然科学基金，安徽省高等学校自然科学基金 |
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| 摘 要： | 目的:研究黄芪药材中黄芪皂苷的提取工艺及含量测定。方法:采用70%乙醇回流提取,水饱和正丁醇萃取,D101大孔吸附树脂富集纯化黄芪总皂苷,硅胶柱层析分离不同的皂苷组分;采用薄层色谱法(TLC)、高效液相-示差检测器法(HPLC-RID)进行皂苷类成分的鉴定和含量测定。结果:从黄芪总皂苷中分离出六种黄芪皂苷化合物(AS-Ⅰ～AS-Ⅵ),其中AS-Ⅰ部分经鉴定主要含皂苷Ⅱ,质量分数为29.4%,AS-Ⅲ部分经鉴定主要含皂苷Ⅳ,质量分数为22.82%。其余4个部分有待于进一步鉴定。结论:此法能较好的分离和纯化黄芪皂苷,TLC和HPLC-RID法操作简便,可作为黄芪皂苷的鉴定和含量测定方法。
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| 关 键 词： | 黄芪皂苷 提取 高效液相色谱法 含量测定 |
| 收稿时间： | 2009-08-18 |
The extraction and determination the contents of astragaloside |
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| WANG Pei-pei,XU Du-juan and XIA Quan. The extraction and determination the contents of astragaloside[J]. China Journal of Experimental Traditional Medical Formulae, 2010, 16(4): 27-30 |
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| Authors: | WANG Pei-pei XU Du-juan XIA Quan |
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| Affiliation: | School of Pharmacy,Anhui Medical University, Hefei 23003,China;Department of Pharmacy, Third-Grade pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine, The First Affiliated Hospital of Anhui Medical University,Hefei 23002,China;Department of Pharmacy, Third-Grade pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine, The First Affiliated Hospital of Anhui Medical University,Hefei 23002,China;Department of Pharmacy, Third-Grade pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine, The First Affiliated Hospital of Anhui Medical University,Hefei 23002,China |
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| Abstract: | |
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| Keywords: | astragaloside extraction HPLC assay |
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