早期电针干预对SAMP8小鼠学习记忆能力和海马磷酸化Tau蛋白水平的影响

目的:探讨早期电针"百会""大椎""肾俞"穴对快速老化小鼠(SAMP8小鼠)学习记忆能力和海马磷酸化Tau蛋白表达的影响,为临床电针治疗阿尔茨海默病(AD)时间点的选择提供参考。方法:将36只3月龄SAMP8小鼠随机分为模型组、3月龄电针组、9月龄电针组,每组12只;以12只同龄正常老化SAMR1小鼠作为空白对照组。3月龄电针组及9月龄电针组分别于小鼠3月龄及9月龄时予电针"百会""大椎""肾俞"穴治疗(连续波,2 Hz,1.5～2 mA),20 min/次,每日1次,8 d为一疗程,疗程间隔2 d,共治疗3个疗程。每组小鼠在水迷宫检测学习记忆能力结束后统一于10月龄取材;免疫组织化学法、免疫蛋白印迹法(Western blot)检测小鼠海马磷酸化Tau蛋白的表达,实时荧光定量PCR法检测小鼠海马Tau mRNA表达。结果:与空白对照组比较,模型组小鼠逃避潜伏期明显延长(P<0.01),原平台象限停留时间较短,跨越平台次数减少(P<0.01),海马磷酸化Tau蛋白及Tau mRNA表达较高(P<0.01);与模型组比较,3月龄电针组及9月龄电针组小鼠逃避潜伏期缩短(P...

Effect of early intervention of electroacupuncture on learning-memory ability and level of hippocampal phosphorylated Tau protein in SAMP8 mice

Objective To explore the effect of early intervention electroacupuncture(EA) at "Baihui"(GV 20), "Dazhui"(GV 14) and "Shenshu"(BL 23) on the learning-memory ability and the expression of phosphorylated Tau protein in the hippocampus of SAMP8 mice, so as to provide reference for the intervening period of EA for Alzheimer′s disease(AD). Methods A total of 36 3-month old SAMP8 mice were randomly divided into a model group, a 3-month-old EA group and a 9-month-old EA group, 12 mice in each group. Twelve normal SAMR1 mice with the same age were taken as the control group. The mice in the 3-month-old EA group and 9-month-old EA group were treated with EA at "Baihui"(GV 20), "Dazhui"(GV 14) and "Shenshu"(BL 23) separately 3 months old and 9 months old(continuous wave, 2 Hz, 1.5-2 mA), 20 min each time, once a day, 8 days as a course of treatment, with an interval of 2 days between courses,totally 3 courses of treatment were given. The mice sample in each group was collected at the age of 10 months after the learning-memory ability tested by Morris water maze. The expression of phosphorylated Tau protein in the hippocampus was detected by immunohistochemistry and Western blot, and the expression of Tau mRNA was detected by real-time PCR. Results Compared with the control group, in the model group, the escape latency was significantly increased(P<0.01), the time of stay in the original platform quadrant and the number of crossing the platform quadrant were reduced(P<0.01), and the expressions of phosphorylated Tau protein and Tau mRNA in hippocampus were increased(P<0.01). Compared with the model group, in the 3-month-old EA group and 9-month-old EA group, the escape latency was significantly reduced(P<0.05), the time of stay in the original platform quadrant and the number of crossing the platform quadrant were increased(P<0.05), and the expressions of phosphorylated Tau protein and Tau mRNA in hippocampus were reduced(P<0.05). Compared with the 9-month-old EA group, in the 3-month-old EA group, the escape latency was significantly reduced(P<0.05), the time of stay in the original platform quadrant and the number of crossing the platform quadrant were increased(P<0.05), and the expressions of phosphorylated Tau protein and Tau mRNA were reduced(P < 0.01). Conclusion The early EA intervention could more effectively improve the learning-memory ability and inhibit phosphorylation of Tau protein in the hippocampus of SAMP8 mice.