通督活血汤对大鼠脊髓损伤后内质网应激信号表达的影响

目的:观察通督活血汤对大鼠脊髓损伤(SCI)后肢体运动功能恢复与内质网应激p-PERK/ATF4/CHOP信号通路表达的影响。方法:56只SD大鼠随机分为假手术组、模型组、实验组,利用改良Allen打击法建立脊髓损伤动物模型,分别于术后1,3,7 d后采用BBB评分和苏木精-伊红(HE)染色观察大鼠后肢功能与脊髓组织形态变化,Western Blot检测p-PERK,ATF4,CHOP表达情况。结果:自干预第3天起,实验组行为学评分优于模型组,差异有统计学意义(P<0.05或P<0.01)。苏木精-伊红染色提示实验组神经细胞水肿较轻,神经细胞形态较好,空泡样改变较少,损伤神经细胞恢复中,部分神经细胞细胞核肿大,核仁消失。Western Blot检测结果示:假手术组p-PERK,ATF4,CHOP表达较低;模型组和实验组p-PERK,ATF4和CHOP在第3天达到高峰;与模型组比较,实验组各时间点p-PERK,ATF4,CHOP的表达显著减少,差异有统计学意义(P<0.05或P<0.01)。结论:通督活血汤治疗SCI的作用机制可能与该方调控SCI后p-PERK/ATF4/CHOP信号表达有关。

Effects of Tongduhuoxue Decoction on Expression of Endoplasmic Reticulum Stress Pathway of Rats with Spinal Cord Injury

Objective:To observe the effects of Tongduhuoxue decoction on limb motor function recovery and expression of endoplasmic reticulum stress related p-PERK/ATF4/CHOP pathway after spinal cord injury(SCI)in rats.Methods:56 SD rats were randomly divided into control group,model group and experimental group.The Allen method was used to establish the animal model.The hind limb function and spinal cord histomorphology were detected by BBB and HE staining at 1,3 and 7 d after intervention.The expressions of p-PERK,ATF4 and CHOP were detected by Western Blot.Results:The behavioral score of the experimental group was better than that of the model group after 3 d intervention(P<0.05 or P<0.01).HE staining revealed that the experimental group had less edema of nerve cells,better morphology of nerve cells and vacuolization with damaged nerve cells recovery,and some nerve cells showed enlarged nucleus and the nucleolus disappears.Western Blot revealed that the expression of p-PERK,ATF4 and CHOP in the sham operation group was lower.The model group and the experimental group p-PERK,ATF4 and CHOP reached the peak values on the 3 rd day.Compared with the model group,the expression of p-PERK,ATF4,CHOP in experimental group were significantly reduced at each time point(P<0.05 or P<0.01).Conclusion:The mechanism of the treatment of SCI by Tongduhuoxue decoction may be related to the expression of p-PERK/ATF4/CHOP pathway after SCI regulation.