四君子汤总多糖对化疗荷瘤小鼠脾脏和派氏结及肠系膜淋巴细胞的影响

目的研究四君子汤总多糖(SJZPS)对化疗荷瘤小鼠脾脏、派氏结(PPs)、肠系膜淋巴结(MLNs)淋巴细胞的影响。方法将BALB/c小鼠随机分为对照组、肿瘤模型组,环磷酰胺(CY)化疗组和四君子汤总多糖治疗(CY+SJZPS)组。CY组和CY+SJZPS组每天腹腔注射CY20 mg.kg-1,CY+SJZPS组同时灌胃SJZPS 1g.kg-1,连续给药9 d。应用流式细胞术检测脾脏、小肠PPs和MLNs中T、B淋巴细胞比例、活化水平以及T细胞亚群的变化。结果 SJZPS能提高肠道PPs中B淋巴细胞的比例和活化水平,降低MLNs中CD4+/CD8+的比值,改善化疗所致的肠道黏膜免疫损伤;SJZPS能提高化疗荷瘤小鼠脾脏淋巴细胞中B淋巴细胞的比例,及T、B淋巴细胞的活化水平,同时降低CD4+/CD8+的比值以改善化疗荷瘤小鼠系统免疫功能,其中SJZPS对B细胞的作用更加明显。结论 SJZPS能有效拮抗化疗药物所致的小鼠肠黏膜免疫功能损伤,同时对整体免疫功能具有改善作用。

Effects of Polysaccharides of Sijunzi Decoction on Lymphocyte in Spleen, Peyer's Patch and Mesenteric Lymph Nodes in Tumor-bearing Mice Tread with Chemotherapy

Objective To explore the effects of polysaccharides of Sijunzi decoction(SJZPS)on lymphocyte in spleen,Peyer’s patchs(PPs) and mesenteric lymph nodes(MLNs) in tumor-bearing mice tread with chemotherapy.Methods BALB/c mice were randomly divided into four groups,control group,tumor model group,cyclophosphamide(CY) chemotherapy group and SJZPS treatmen(t CY+SJZPS)group.CY group and CY+SJZPS group daily intraperitoneal injection administration of cyclophosphamide(20 mg.kg-)1,CY+SJZPS group daily oral administration of SJZPS(1 g.kg-1)at the same time.The proportion and activation level of(T,B)lymphocyte,subsets of T lymphocyte isolated from spleen,PPs and MLNs were detected by flow cytometer.Results In CY+SJZPS group,the intestine mucosal immunity injury induced by cyclophosphamide was improved by increasing the proportion and activation level of B lymphocyte in PPs,decreasing the ratio of CD4+/CD8+ in MLNs,and the systematic immunity was improved by increasing the proportion of B cell and the activation level of T、B cell,and decreasing the ratio of CD4+/CD8+ of spleen lymphocyte in tumor-bearing mice tread with chemotherapy.Conclusion Polysaccharides of Sijunzi decoction can effectively antagonize the injury on intestine mucosal immunity induced by cyclophosphamide in tumor-bearing mice,and can improve the systematic immunity.