气相色谱法测定喉康散中龙脑与薄荷脑的含量

目的建立以气相色谱法测定喉康散中龙脑和薄荷脑含量的方法。方法色谱柱为Supel CO WAX-10石英毛细管色谱柱(30 m×0.32 mm,0.5μm),载气为N2,检测器为FID,进样口温度为200℃,检测器温度为250℃,内标物为水杨酸甲酯。结果龙脑在0.02975～0.47701 mg·mL-(1r=0.999949),薄荷脑在0.04824～0.77083 mg·mL-(1r=0.99998)范围内同各自峰面积与内标峰面积比值呈良好的线性关系;二者回收率分别为100.77%和101.62%,RSD分别为1.35%和1.38%(n=6)。结论本法操作简便、快速,结果准确、可靠,可用于喉康散的质量控制。

Determination of Borneol and Menthol in Houkang Powder by Gas Chromatography

Objective To establish the gas chromatography(GC)method for the determination of borneol and menthol in Houkang Powder.Methods The determination was performed on Supelco wax-10(30 m×0.32 mm,0.5 μm) column with nitrogen as carrier gas and FID as detector.the inlet temperature was 200 ℃.The injector temperature was kept at 250 ℃ with methyl salicylate as internal standard.Results The linear range of borneol was 0.02975 to 0.47701 mg·mL-(1r=0.9999) with average recovery of 100.77 %(RSD=1.35 %,n=6).The linear range of menthol was 0.04824 to 0.77083 mg·mL-1(r=0.9999) with average recovery of 101.62 %(RSD =1.38 %,n=6).Conclusion This method is simple,rapid,accurate and reliable for the quality control of Houkang Powder.