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| 反相高效液相色谱法测定复方硫酸氨基葡萄糖分散片的含量 | |
| 引用本文： | 初立梅,徐健峰,石涛,徐康康. 反相高效液相色谱法测定复方硫酸氨基葡萄糖分散片的含量[J]. 中国药业, 2010, 19(5): 20-21 |
| 作者姓名： | 初立梅 徐健峰 石涛 徐康康 |
| 作者单位： | 1. 南京医科大学附属南京儿童医院,江苏,南京,210008 2. 南京威尔曼药物研究所,江苏,南京,210009 3. 东南大学医学院附属中大医院,江苏,南京,210009 |
| 摘 要： | 目的建立硫酸氨基葡萄糖含量测定的高效液相色谱（HPLC）法。方法样品柱前衍生化后采用反相高效液相色谱（RP—HPLC）进行测定，色谱柱为Hypersil ODS柱（50mm×4．6mm，5μm），流动相为三水舍乙酸钠水溶液-甲醇（90：10），用紫外检测器检测。结果方法的进样量线性范围为300—1500μg／mL，r=0．9997（n=5），平均回收率为102．68％，RSD=1．08％（n=9）。结论此法简便、快速、准确，是测定硫酸氨基葡萄糖含量的有效方法。 |
| 关 键 词： | 衍生化 反相高效液相色谱法 硫酸氨基葡萄糖 含量 |
Determination of Glucosamine Sulphate Dispersible Tablets by Pre- Column Derivatization RP- HPLC Method |
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| Chu Limei,Xu Jianfeng,Shi Tao,Xu Kangkang. Determination of Glucosamine Sulphate Dispersible Tablets by Pre- Column Derivatization RP- HPLC Method[J]. China Pharmaceuticals, 2010, 19(5): 20-21 | |
| Authors: | Chu Limei Xu Jianfeng Shi Tao Xu Kangkang |
| Affiliation: | 1. Nanjing Children＇s Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, China 210008; 2. Nanfing Welman Institute of Pharmacology, Nanjing, Jiangsu, China 210009; 3. Zhongda Hospital Affiliated to Southeast University, Nanjing, Jiangsu, China 210009) |
| Abstract: | Objective To establish a HPLC method for the determination of glucosamine sulphate. Methods The sample of glucosamine sul- phate was treated by pre-column derivatization, then determined by RP-HPLC on the Hypersil ODS column （50 mm ×4. 6 mm,5 μm） with CH3COONa.3H2O - CH3OH（90 ： 10） as the mobile phase and detected by the UV detector. Results The linear range of calibration curve was 300-1 500 μg/mL, r=0. 9997（n=5）.The recovery rate was 102.68%, RSD=l.08% （n=9）.Conclusion The method is simple, rapid and accurate, which is an effective method for the determination of glucosamine sulphate. |
| Keywords: | derivatization RP - HPLC glucosamine sulphate content |
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