| Zhichan decoction induces differentiation of dopaminergic neurons in Parkinson’s disease rats after neural stem cell transplantation |
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| 作者单位： | [1]Department of Neurology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai, China [2]Department of Encephalopathy, Liu＇an Hospital of Traditional Chinese Medicine, Liu＇an, Anhui Province, China [3]College of Acupuncture and Massage, Shanghai University of Traditional Chinese Medicine, Shanghai, China |
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| 基金项目： | financially supported by the National Natural Science Foundation of China,No.30772870 |
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| 摘 要： | The goal of this study was to increase the dopamine content and reduce dopaminergic metabolites in the brain of Parkinson’s disease rats. Using high-performance liquid chromatography, we found that dopamine and dopaminergic metabolite（dihydroxyphenylacetic acid and homovanillic acid） content in the midbrain of Parkinson’s disease rats was increased after neural stem cell transplantation ＋ Zhichan decoction, compared with neural stem cell transplantation alone. Our genetic algorithm results show that dihydroxyphenylacetic acid and homovanillic acid levels achieve global optimization. Neural stem cell transplantation ＋ Zhichan decoction increased dihydroxyphenylacetic acid levels up to 10-fold, while transplantation alone resulted in a 3-fold increment. Homovanillic acid levels showed no apparent change. Our experimental findings show that after neural stem cell transplantation in Parkinson’s disease rats, Zhichan decoction can promote differentiation of neural stem cells into dopaminergic neurons.
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| 关 键 词： | 神经干细胞移植 多巴胺能神经元 细胞分化 大鼠脑 羟基苯乙酸 高效液相色谱法 金 诱导 |
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