D-半乳糖亚急性中毒大鼠拟衰老生理生化改变

目的　D 半乳糖皮下注射致Wistar大鼠亚急性衰老模型 ,观察大鼠衰老指标的变化。方法　 2 0只雄性大鼠随机分为 2组 ,每组 10只。对照组每日一次皮下注射生理盐水 10ml kg,衰老模型组每日一次皮下注射D 半乳糖 4 8mg kg。各组大鼠均连续给药 4 2d ,采血后分别测定各项衰老指标。结果　与对照组比较 ,衰老模型组体重增加比例降低 ,胸腺明显萎缩 ,脏器系数减少 (P <0 .0 1) ,血清中超氧化物歧化酶 (SOD)活性明显下降 (P <0 .0 1)。结论　D 半乳糖诱发的致大鼠亚急性衰老是探讨自由基衰老学说有用的实验动物造模技术。

Physiologic and Biochemical Changes of Mimetic Aging Induced by D-galactose in Rats

Objective To observe the changes of aging data of Wistar rats in the subacute aging model induced by the subcutaneous injection with D-galactose.Methods 20 male Wistar rats were divided randomly into two groups.Each group contained 10 rats for the control or aging model.The control rats were injected subcutaneously with 10 ml PBS/kg each day,and the rats of the aging model with 48 mg D-galactose/kg each day.After 42 days with continuous injection,the blood specimens were collected for the detection of the data relative to the aging. Results Compared with the control , the weight of the aging model rats increased slowly. The thymus of these rats shrivelled clearly,and their weight decreased as well (P<0.01).In addition,the superoxide dismutase(SOD) activity in the serum of these aging rats declined obviously(P<0.01). Conclusion The aging model could be established by the mimic aging reaction induced with D-galactose.