高压氧用于兔椎动脉型颈椎病致感音神经性耳聋治疗的实验研究

目的:探讨高压氧疗法(HBOT)对椎动脉型颈椎病所致感音神经性耳聋的疗效及其可能作用机制。方法:采用C2—C6左侧横突软组织注射组织硬化剂法构建家兔椎动脉型颈椎病动物模型。实验动物随机分为对照组、模型组和HBOT组。HBOT组从造模第6周开始采用2.2ATA,100%O2,每日1次,连续2周HBOT治疗。测定各组动物50Hz高刺激率、10Hz低刺激率左侧短声听性脑干反应(ABR)。结果:在50Hz高刺激率短声刺激下,模型组ABR的Ⅲ、Ⅳ波峰潜伏期(PL)分别延长至(3.21±0.19)ms、(4.12±0.24)ms,Ⅰ-Ⅳ波峰间期(IPL)至(2.61±0.26)ms,与对照组比较差异具有显著性(P<0.05),Ⅰ-Ⅲ波IPL延长至(1.69±0.22)ms,与对照组比较差异非常显著(P<0.01);HBOT使Ⅲ、Ⅳ波PL分别缩短为(2.99±0.21)ms、(3.90±0.20)ms,Ⅰ-Ⅲ波IPL为(1.49±0.19)ms,与模型组比较差异具有显著性(P<0.05);各组间10Hz低刺激率ABR差异无显著性意义。结论:HBOT可改善颈源性感音神经性耳聋的脑干神经元突触效能和外周听觉通路传导,恢复听觉功能。

Experimental study of hyperbaric oxygen therapy on sensorineural hearing loss induced by vertebroarterial type cervical spondylosis

Objective:To evaluate the effect of hyperbaric oxygen therapy(HBOT) on sensorineural hearing loss induced by vertebroarterial type cervical spondylosis and its possible mechanism.Method:The animal model of vertebroarterial type cervical spondylosis was established by injection sclerosant into the soft tissue on the left transverse processes of C2—C6 of rabbit.Thirty rabbits were randomly divided into 3 groups:control group,model group and HBOT group.In HBOT group,HBOT was administered by using 100% oxygen at 2.2 ATA once daily for 2 weeks from the 6th week after injection.The auditory brainstem response(ABR) evoked by clicking at high(50Hz) or low(10Hz) stimulation rate were recorded from the left ear of rabbit in each group.Result:At 50Hz high stimulation rate in model group,the record of ABR showed the peak latency(PL) of Ⅲ and Ⅳ wave were(3.21±0.19)ms and(4.12±0.24)ms respectively,and the interpeak latency(IPL) of Ⅰ-Ⅵ and Ⅰ-Ⅲ wave were(2.61±0.26)ms and(1.69±0.22)ms respectively,longer than those in control group(P<0.05;P<0.01).While in HBOT group PL of Ⅲ and Ⅳ wave and IPL of Ⅰ-Ⅲ wave were(2.99±0.21)ms,(3.90±0.20)ms and(1.49±0.19)ms respectively,compared with those in model group,the differences were significant(P<0.05).At 10Hz low stimulation rate there was no significant difference in PL or IPL of ABR among three groups.Conclusion:HBOT can improve not only the neuron synapses efficiency of brainstem,but also the conduction of peripheral cochlear path.This may be the cause for HBOT improving the hearing function in sensorineural hearing loss.