多聚赖氨酸-硅纳米颗粒的生物相容性研究

背景与目的：多聚赖氨酸-硅纳米颗粒是一种新型的非病毒纳米颗粒基因传递载体。本研究主要阐述其生物相容性,为其进一步的体内应用提供实验依据。方法：细胞转染和流式细胞仪分析在含血清培养基中多聚赖氨酸-硅纳米颗粒介导质粒DNA和反义寡核苷酸的转染能力,随后通过血浆蛋白反应滤过试验和红细胞聚集试验进一步评价其生物相容性。结果：在含血清培养基中,多聚赖氨酸-硅纳米颗粒介导质粒DNA和反义寡核苷酸传递的能力显著下降。多聚赖氨酸-硅纳米颗粒／DNA(／ODN)复合物可与小鼠血浆蛋白成分结合,并可引起红细胞聚集。结论：多聚赖氨酸-硅纳米颗粒可能与含血清培养基中的血浆蛋白相互作用,影响体外细胞转染效率。多聚赖氨酸-硅纳米颗粒可能与体内外周血血浆蛋白和外周血红细胞反应而影响其体内基因传递能力,其生物相容性还有待于进一步提高。

Biocompatibility of poly-l-lysine-modified silica nanoparticles

BACKGROUND &OBJECTIVE:Poly l lysine modified silica nanoparticle(PMS NP) was a novel non viral vector for gene delivery. The current study was designed to evaluate the biocompatibility of PMS NP for its further utilization in vivo. METHODS: Cell transfection and flow cytometry were used to elucidate the delivery efficiency of plasmid DNA and antisense ODN mediated by PMS NP in the presence of serum containing medium. Subsequently, the biocompatibility of PMS NP in vivo was evaluated using filtration assay of plasma proteins and erythrocyte aggregation assay. RESULTS: The abilities of PMS NP to deliver plasmid DNA and antisense ODN in vitro clearly decreased in the presence of serum containing medium. PMS NP/DNA(ODN)complexes bound plasma proteins and triggered erythrocyte aggregation. CONCLUSION: PMS NP might interact with plasma proteins, resulting in decreased transfection efficiency in vitro. And filtration assay of plasma proteins and the erythrocyte aggregation assay demonstrated that the interaction of PMS NP with plasma proteins and erythrocytes might play a negative role in gene transfection efficiency in vivo. And its biocompatibility needs to be further improved.