灵芝孢子粉对裸鼠移植性人肝肿瘤血管生成的抑制作用

目的研究灵芝孢子粉对裸鼠移植性人肝肿瘤血管生成的影响，并初步探讨其抑瘤机制。方法建立裸鼠移植性人肝肿瘤模型，采用随机区组设计法随机分为空白对照组、阳性对照组及灵芝孢子粉组，观察裸鼠肿瘤生长。采用苏木精-伊红染色法、SP免疫组织化学法及病理Imagepro Plus5．0彩色图像定量分析法检测裸鼠肿瘤组织中血管内皮生长因子（VEGF）和微血管密度（MVD）。结果灵芝孢子粉为2．1g／kg时，对裸鼠移植瘤的抑制率为57．0％，与空白对照组比较有显著差异（P〈0．01）。镜下观察，灵芝孢子粉组与空白对照组相比；肿瘤坏死组织较多，细胞异型性较小。灵芝孢子粉组VEGF、MVD表达水平较空白对照组有明显下降。结论高浓度及大剂量的灵萝孢子粉对肝肿瘤有明展抑制作用．并能抑制肿瘤新生血管生成．其机制可能与对VEGF表达的抑制有关。

Inhibitory and antiangiogenetic effects of Lucid Ganoderma spore on the human hepatocarcinoma transplanted in nude mice

Objective To evaluate the inhibitory effect and antiangiogenetic effect of Lucid Ganoderma spore(LGS) on the growth of the human hepatocarcinoma HepG_2 xenograft in athymic nude mice.Methods The nude mice models of human hepatocarcinoma HepG_2 were established and randomly divided into a NS blank control group,a 5-FU positive treatment control group and a LGS treatment group.H-E staining,SP immunohistochemistry and quantitative pathologic image analysis system were used to investigate the microvessel density(MVD) and expression of vascular endothelial growth factor(VEGF) in tumor tissue.Results The in vivo study revealed that LGS produced quite certain therapeutic effect on the growth of the hepatocarcinoma cell strain(dose:2 g/kg,inhibitory rate:57.0%,P<0.01).Morphologic study showed there were more necrosis and less degree of atypia,decreased MVD and VEGF expression in the LGS group,as compared with that in the blank control group.Conclusion LGS could inhibit the growth and angiogenesis of the transplanted tumor tissue of human hepatocarcinoma HepG_2 in nude mice.Its mechanisms may be related to the reduction of VEGF expression.