裸鼠胆囊癌皮下移植瘤超声造影定量参数与血管生成及细胞增殖的相关性

目的 探讨裸鼠胆囊癌皮下移植瘤CEUS定量参数与肿瘤血管生成和细胞增殖状态的相关性。方法 建立裸鼠胆囊癌皮下移植瘤模型，测定CEUS定量参数和微血管密度（MVD）、血管内皮生长因子（VEGF）及增殖细胞核抗原（PCNA）的表达，并进行相关性分析。结果 成功建立移植瘤20个，最终CEUS成功16个。裸鼠胆囊癌皮下移植瘤PI与MVD、PCNA呈正相关（r=0.635、0.514，P=0.008、0.042），而CEUS各定量参数与VEGF表达均无相关性（P均>0.05）。裸鼠胆囊癌皮下移植瘤MVD与VEGF、PCNA表达呈正相关（r=0.680、0.650，P=0.004、0.006），VEGF与PCNA呈正相关（r=0.611，P=0.012）。结论 裸鼠胆囊癌皮下移植瘤PI与MVD、PCNA呈正相关，有助于评估肿瘤的血管生成及细胞增殖状态；MVD、VEGF、PCNA两两之间均呈正相关，表明肿瘤血管生成与细胞增殖之间相互促进，共同促进肿瘤生长。

Correlation between CEUS parameters and angiogenesis and cell proliferation of gallbladder carcinoma transplant tumor in nude mice

Objective To explore the correlation between CEUS blood perfusion characteristics and angiogenesis and cell proliferation in nude mice models of gallbladder carcinoma transplant tumor. Methods Nude mice models of gallbladder carcinoma transplant tumor were established. The blood perfusion characteristics of the nude mice models were quantitatively analyzed, CEUS quantitative parameters and the expression levels of microvessel density (MVD), vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) immunohistochemically were obtained. The correlations were performed. Results Twenty tumors were successfully established. The CEUS of 16 tumors was successful. There was positive correlation between peak intensity (PI) and MVD, PCNA (r=0.635, 0.514, P=0.008, 0.042). The CEUS parameters were not correlated with expression of VEGF. MVD had positive correlation with expression of VEGF, PCNA (r=0.680, 0.650, P=0.004, 0.006); VEGF had positive correlation with expression of PCNA (r=0.611, P=0.012). Conclusion The PI of CEUS is positive correlation with MVD and PCNA in nude mice models of gallbladder carcinoma transplant tumor, which may be helpful to evaluate tumor angiogenesis and cell proliferation state. The MVD, VEGF and PCNA positively correlates to each other, which indicates there is the relationship of reciprocal promotion between tumor angiogenesis and cell proliferation, and is good for tumor growth.