2型糖尿病大鼠肾脏活性氧物质与NO关系的探讨

目的研究糖尿病大鼠肾脏活性氧物质与一氧化氮(NO)系统的关系。方法制备高脂高糖加小剂量链脲佐菌素(35mg/kg)诱导的2型糖尿病大鼠模型。将大鼠分为正常对照组10只和糖尿病组20只;观察糖尿病大鼠肾脏氧化应激状态及NO系统的表达,肾功能改变及抗氧化指标与NO的相关性分析。结果糖尿病大鼠成型12周时SOD,SOD/MDA降低,MDA升高,与正常对照组比较差异有统计学意义;NO、总NOS、iNOS、cNOS均较正常对照组明显升高。肾脏肥大指数、尿微量清蛋白、基质比、肾小球截面积与SOD/MDA呈负相关,与NO、总NOS呈正相关;SOD/MDA与总NOS、NO之间存在负相关。结论氧化应激,NO均参与糖尿病肾病的发生、发展,它们之间的平衡可能是抗氧化治疗在糖尿病肾病中的关键。

The relation between the reactive oxygen species and nitric oxide of diabetic nephropathy in type 2 diabetic rats

Objective To study the relation between the reactive oxygen species and nitric oxide(NO) of diabetic nephropathy in type 2 diabetic rats.Methods Making type 2 diabetic rats by high-sucrose-high-fat diet with a low dose of STZ(35 mg/kg) injecting into abdominal cavity.The rats divided into control group(n=10) and diabetic group(n=20).The expression of oxidative stress and Nitric Oxide system in diabetic nephropathy were observed and the correlation of oxidative index,Nitric Oxide and renal function was analyzed.Results In diabetic rats,SOD,SOD/MDA were reduced in comparison with control group while MDA was increased.NO,Total NOS,iNOS,cNOS were elevated in diabetic group;the kidney weight/body weight,urine microalbumin,mean glomerular area,mean mesangial matrix area/mean glomerular area ratio negatively correlated with SOD/MDA,while positively correlated with NO,total NOS;SOD/MDA negatively correlated with NO,total NOS.Conclusion The oxidative stress and NO participate in the diabetic nephropathy,the balance is the key point that the treatment of antioxidant in the diabetic nephropathy probably.