金雀异黄素抑制大鼠膀胱肿瘤作用的实验研究

目的探讨金雀异黄素对N-甲基亚硝基脲(MNU)诱导的SD大鼠膀胱肿瘤的抑制作用。方法将60只大鼠随机分成3组,用MNU诱导成膀胱肿瘤,实验1组和实验2组分别经腹腔注射金雀异黄素0.5、1.0mg/kg,每两日1次;对照组用同样的方式注射生理盐水。利用统计方法和病理分级来分析金雀异黄素对MNU诱导的SD大鼠膀胱肿瘤的抑制作用。结果实验1组和实验2组与对照组比较,在体质量减少值、肿瘤个数、抑瘤率等方面,差异有统计学意义(P<0.05);实验1组和实验2组的癌变率相比,差异有统计学意义(P<0.05)。结论金雀异黄素对大鼠膀胱肿瘤有抑制作用,剂量越大,对肿瘤癌变抑制作用越强。

The experimental study about genistein inhibition of rats bladder tumor

Objective Investigate the effects about genistein in inhibition of N-methyl nitrosourea(MNU)-induced SD rats bladder tumor.Methods 60 SD rats were divided into 3 groups randomly,and all of the 3 groups were treated with MNU to induce urinary bladder tumor.Experimental group1 and 2,respectively,by intraperitoneal injection of genistein of 0.5,1.0 mg/kg,every 2 days,the same way injection of saline control group.The statistical methods and pathology classification were used to analyze the effect of genistein on MNU-induced SD rat bladder tumor inhibition.Results Compared with control group,in four aspects which were eight loss value,tumour number,inhibition rate of tumor and pathology classification,the difference was statistically significant(P<0.05).Experimental group 1 and experimental group 2 compared to rate of cancer,the difference was statistically significant(P<0.05).Conclusion Genistein inhibited rats bladder tumor,the greater the dose,the stronger the inhibition of tumor cancer.