高压氧结合电针治疗对脊髓损伤后大鼠膀胱功能的影响

目的观察高压氧结合电针治疗对脊髓损伤(SCI)后大鼠神经源性膀胱功能的影响及机制分析。方法将雌性SD大鼠60只,随机分为对照组、高压氧组及高压氧结合电针组(结合电针组),每组20只。所有大鼠用Allen′s重物撞击法制造SCI模型,其中对照组不接受任何治疗,高压氧组及结合电针组在造模成功12h后每天接受高压氧治疗或高压氧结合电针治疗1次。各组大鼠分别于术后24h,3、7、14d在乌拉坦麻醉下尿道置管行尿流动力学检查后处死。观察比较3组大鼠脊髓中及膀胱组织中神经型一氧化氮合酶(nNOS)表达及尿流动力学指标。结果在各时间点上,高压氧组及结合电针组脊髓及膀胱组织中nNOS表达明显高于对照组(P<0.01),结合电针组脊髓及膀胱组织中nNOS表达明显高于高压氧组(P<0.05);尿流动力学结果结合电针组显著优于高压氧组及对照组(P<0.05)。结论高压氧结合电针治疗对SCI后大鼠的神经元具有保护作用,并能很好改善大鼠的膀胱功能。

Effects of hyperbaric oxygen combined electroacupuncture on bladder function in SD rats after spinal cord injury

Objective To study the effects of hyperbaric oxygen(HBO) combined electroacupuncture on the bladder function in SD rats after spinal cord injury(SCI).Methods 60 SD rats were randomized into 3 groups:control group(n=20),HBO group(n=20) and HBO combined electroacupuncture group(n=20).All rats were constructed the SCI model by adopting Allen′s heavy dropping method,in which none of treatment was given to the control group,while the other two groups accepted the HBO therapy or HBO combined electroacupuncture once per day after 12 h of establishing model.Rats in various groups were performed the urodynamic examination under urethane anesthesia at 24 h,3,7,14 d after operation and then killed.Expression of neuronal nitric oxide synthase(nNOS) and the urodynamic indexes were observed.Results In each time point,the nNOS expression in the tissues of spinal cord and bladder in the HBO combined electro acupuncture group and the HBO group were significantly higher than that in the control group,and the nNOS expression in the HBO combined electroacupuncture group was obviously higher than that in the HBO group.The urodynamic results showed that the HBO combined electroacupuncture group was significantly superior to the HBO group and the control group(P<0.05).Conclusion HBO combined electroacupuncture could protect the neuron cells and improve the bladder function greatly after SCI in rats.