低频超声对大鼠颊囊黏膜溃疡组织SOD、MDA含量的影响

目的探讨不同功率低频超声处理实验性SD大鼠颊囊黏膜溃疡组织中超氧化物歧化酶(SOD)、丙二醛(MDA)含量的影响。方法将36只健康SD大鼠随机分为对照组(n=6)及大鼠颊囊黏膜溃疡模型组(n=30);溃疡模型组根据不同处理方式分为溃疡组(n=6),药物组(n=6)及低频超声组(n=18),低频超声组大鼠按超声波输出功率再分为1.10、1.58、2.06 W组,每组6只。治疗结束24h后观察各组大鼠溃疡的愈合时间;制备溃疡组织匀浆,采用分光光度计测定大鼠颊囊黏膜溃疡组织中SOD、MDA含量。结果药物组及低频超声组大鼠颊囊黏膜溃疡的平均愈合时间均比溃疡组短(P<0.05),但不同功率低频超声组大鼠颊囊黏膜溃疡的愈合时间比较,差异无统计学意义(P>0.05)。处理结束24h后,低频超声组大鼠颊囊黏膜组织中SOD含量较溃疡组显著升高(P<0.05),而其与对照组、药物组比较差异无统计学意义(P>0.05),溃疡组中SOD含量较对照组显著降低(P<0.05);低频超声组中MDA含量较溃疡组显著降低(P<0.05),与对照组、药物组比较差异无统计学意义(P>0.05),溃疡组中MDA含量较对照组显著升高(P<0.05)。结论安全范围输出功率的脉冲低频超声能有效升高组织中SOD活性,降低MDA含量,对溃疡的愈合有一定的促进作用。

Effects of low-frequency ultrasound irradiation on SOD and MDA levels in cheek pouch mucosa ulcer tissue of rats

Objective To observe the effects of different energy levels of low-frequency ultrasound irradiation on the changes of SOD and MDA levels in ulcer tissue of rat oral ulcer model.Methods Thirty Sprague-Daw-ley rats were inflicted with oral ulcer,and then randomly and equally assigned to 5 groups,oral ulcer group,1% iodine glycerin group and the rest 3 groups receiving low-frequency ultrasound irradiation at 1.1,1.58,2.06 W(once per day,for consecutive 3 d).The other 6 rats served as normal control.In 24 h after last treatment,3 rats from each group were sacrificed and their cheek pouches were taken out for detecting SOD and MDA levels in the mucosal tissue by spectrophotometry.Ulcer healing was observed at 24 h after the last treatment for consecutive 8 d.The efficiency of low-frequency ultrasound irradiation at different powers was evaluated.Results The level of SOD in 1.1,1.58,2.06 W groups was significantly higher than that of the ulcer group(P<0.05).The level of MDA in 1.1,1.58,2.06 W groups was significantly lower than that of the ulcer group(P<0.05),but the level of SOD and MDA had no significant difference(P>0.05) between the 1.1,1.58,2.06 W groups and the control group.Conclusion Low-frequency ultrasound in safe range of output power increases the SOD activity and decreases the MDA content effectively,and has certain promoting effect on ulcer healing.