糖尿病大鼠胰岛B细胞损伤的形态定量研究

目的探讨胰岛B细胞损伤后数量能否自行恢复。方法雄性SD大鼠分2批进行实验,2批均随机分为对照组和实验组。实验组按60mg/kg一次性腹腔内注射链脲佐菌素(STZ),对照组注射等量的缓冲液;在注射前和注射后的第7天(第1批和第2批)和第84天(第2批),检测空腹血糖;在第7天(第1批)和第84天(第2批)同时检测血清胰岛素,并取胰腺行胰岛素免疫组织化学染色;用体视学形态定量研究方法测量胰岛总体积、胰岛总面积和单位面积胰岛内B细胞核数量等。结果与对照组的胰岛总体积、胰岛总面积、单位面积胰岛内B细胞核数量、血清胰岛素和空腹血糖比较,成模大鼠在注射STZ后第7天和第84天差异均有统计学意义。成模大鼠的胰岛总体积、胰岛总面积和单位面积胰岛内B细胞核数量在第7天和第84天差异均无统计学意义;血清胰岛素在第84天比第7天减少了71%(P<0.05);空腹血糖在第84天比第7天升高了30%(P<0.05)。结论胰岛B细胞损伤后,随着病程的延长,其数量不能自行恢复。

Study of the damage of pancreas islet B-cell in diabetic rats using morphometry

Objective To explore whether pancreas islet B-cell damaged can reverse spontaneously.Methods There were two experiments in which SD rats were divided into control group and experimental group.Rats in the experimental group received intraperitoneal injection of 60 mg/kg STZ and rats in the control group received intraperitoneal injection of equal dose acidified saline.At day before injection,7 day(the first and the second experiment) and 84 day(the second experiment) after injection,observation on fasting blood glucose was performed.At 7 day(the first experiment)and 84 day(the second experiment),measurement of serum insulin was performed,the pancreata were removed after excision and the insulin immunohistochemistry of pancreas sections was performed.Measure the total volume,the total area of the pancreas islet and the numbers of B-cell nucleus of unit of area in pancreas islet using stereological methods.Results There were statistical differences in the total volume,the total area of the pancreas islet,the numbers of B-cell nucleus of unit of area in pancreas islet,serum insulin and fasting blood glucose levels between the control and the diabetic group at 7 day and 84 day after injection.There were no statistical differences in the total volume,the total area of the pancreas islet and the numbers of B-cell nucleus of unit of area in pancreas islet between the diabetic group at 7 day and the diabetic group at 84 day after injection.Compared with the diabetic group at 7 day,serum insulin levels was decreased 71%(P<0.05)and fasting blood glucose levels was increased 30%(P<0.05)in the diabetic group at 84 day.Conclusion Pancreas islet B-cell damaged can’t reverse spontaneously when diabetes occurring.