Rapid detection of antigen-specific mouse IgE by enzyme-linked immunosorbent assay (ELISA) |
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| Authors: | R J Strouse D W Anderson D C Argentieri |
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| Affiliation: | R.W. Johnson Pharmaceutical Research Institute, Department of Immunopharmacology Raritan, New Jersey 08869. |
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| Abstract: | A rapid, sensitive, antigen-specific mouse IgE capture ELISA is described. A monoclonal rat anti-mouse IgE was used as the capture antibody, and a DNP-coupled BSA-biotinylated conjugate along with a peroxidase-avidin-biotin complex was utilized as the detection system. The lower detection limit of this assay is 8.5 ng/ml of antigen-specific IgE. With some modifications, this assay can be employed to screen for antigen specific antibodies of other isotypes and subtypes. |
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