冰毒对HIV-1在巨噬细胞内复制影响的体外实验研究

目的探讨冰毒对人类免疫缺陷病毒-1型（human immunodeficiency virus 1，HIV-1）在巨噬细胞中复制的作用。方法将巨噬细胞按单纯随机分组原则分为：（1）冰毒＋多巴胺受体D1阻滞剂（SCH23390）处理组，（2）冰毒处理组，（3）SCH23390处理组，（4）病毒对照组。先用浓度为10-5mol／L的SCH23390预处理（1）、（3）组1h，再用10μmol／L的冰毒处理（1）组24h，同时用10～mol／L、10μmol／L及2．5×10-4mol／L的冰毒处理（2）组24h，然后每组加入等量的HIV-1进行感染，于感染的第4、6、8、10d取培养上清，用酶联免疫吸附剂测定（enzymelinked immunosorbnent assay，ELISA）检测培养上清的HIV-1 p24抗原，比较各组之间抗原表达量差异。结果HIV-1感染巨噬细胞第4、6、8、10d，病毒对照组的p24抗原表达量均低于3个不同浓度冰毒处理组（均有P〈0．01）；且各浓度冰毒处理组的p24抗原表达量比对照组增加的倍数，随着感染时间的延长而具有上升的趋势，时间．效应差异有统计学意义（F=155．94，P〈0．001）；而冰毒＋SCH23390处理组、SCH23390处理组分别与病毒对照组HIV-1p24抗原表达比较，差异均无统计学意义（均有P〉0．05）。结论冰毒能够促进HIV-1在巨噬细胞内的复制，并随感染时间的延长呈递增趋势；冰毒促进HIV-1复制的作用可被多巴胺受体D1阻滞剂（SCH23390）阻断。

Study on the effect of methamphetamine on the HIV-1 replication in macrophages in vitro

Objective To determine the effect of methamphetamine on the replication of HIV-1 in macrophages. Methods Maerophages were assigned randomly into four groups： （ 1 ） the methamphetamine/SCH 23390 co-treatment group ; （2） the methamphetamine treatment group ; （3） the SCH 23390 treatment group ; （4） the control group. Firstly, （1） and（3 ）group were treated with 10-5 mol/L of SCH 23390 for 1 hour. Secondly, （1） group were treated with 10-4 mol/L of methamphetamine for 24 hours and （2） group were treated with 10 -5mol/L, 10 -4mol/L and 2. 5 × 10 -4mol/L of methamphetamine for 24 hours. And then all groups were infected with equal amounts of HIV-1 strain. HIV-1 p24 antigen in culture supernatants collected at days 4, 6, 8 and 10 after infection were tested and then the difference between the anti- gen expressions of the groups were compared. Results On the 4th day,6th day, 8th day and 10th day of macrophages in- fected with HIV-1, the expressions of p24 antigen in the control group were significantly decreased compared to the three different concentrations of methamphetamine treatment group （ all P 〈 0.01 ） ; The increased folds of p24 antigen expressions in different concentrations of methamphetamine-treated groups compared with that of the control group, appeared to have an increasing trend with HIV-1 infected time. This enhanced result showed a significant time-course effect （F = 155.94 ,P 〈 0. 001 ）; There was no significant difference between the methamphetamine/SCH 23390 co-treatment group, the SCH 23390 treatment group and the control group （ all P 〉 0.05 ）. Conclusions Methamphetamine has the ability to enhance HIV-1 replication in macrophages, and this enhanced result shows an increasing trend with HIV-1 infected time; The effect of methamphetamine on promotion of HIV-1 replication can be inhibited by the dopamine D1 receptor antagonists.