| 特异siRNA沉默Slug基因抑制体外胰腺癌AsPC-1细胞生长的实验研究 |
| |
| 引用本文： | 林晶,严明总.特异siRNA沉默Slug基因抑制体外胰腺癌AsPC-1细胞生长的实验研究[J].海南医学院学报,2009,15(7):698-701. |
| |
| 作者姓名： | 林晶 严明总 |
| |
| 作者单位： | 1. 海南省海口市府城医院普外科,海南,海口,571101
2. 海南省人民医院检验科,海南,海口,570311 |
| |
| 基金项目： | 海南医学院科研基金资助学报项目 |
| |
| 摘 要： | 目的：研究Slug基因沉默对胰腺癌AsPC-1细胞增殖、凋亡的影响。方法：构建靶向抑制Slug的siRNA表达载体，瞬时转染AsPC-1细胞。采用RT—PCR和Western blot法检测细胞Slug蛋白，MTT检测细胞增殖，Annexin V-FITC／PI了解细胞凋亡的变化。结果：成功构建了pGenesil-1-Slug-siRNA（pSlug-siRNA）和pCenesil-1-Neg-siRNA（pNeg-siRNA）表达质粒。pSlug-siRNA瞬时转染AsPC-1细胞72h后，细胞S1ug表达及生长受到明显抑制，细胞凋亡率明显增加。结论：Slug-siRNA抑制Slug表达，促进As-PC-1细胞凋亡并抑制细胞的增殖。
|
| 关 键 词： | Slug 小干扰RNA 瞬时转染 增殖 凋亡 胰腺肿瘤 AsPC-1 |
Effects of Slug gene silence by siRNA on proliferation and apoptosis of AsPC-1 in vitro |
| |
| LIN Jing,YAN Ming-zong.Effects of Slug gene silence by siRNA on proliferation and apoptosis of AsPC-1 in vitro[J].Journal of Hainan Medical College,2009,15(7):698-701. |
| |
| Authors: | LIN Jing YAN Ming-zong |
| |
| Institution: | LIN Jing1,YAN Ming-zong2(1.Department of General Surgery,Fucheng Hospital HaiKou 571101,2.Lab Department,People's Hospital in Hainan Province Haikou517000,China) |
| |
| Abstract: | Objective:To inhibit Slug expression in pancreatic cancer cells with small interfering RNA(siRNA),and explore its effect on cell proliferation and apoptosis.Methods:Plasmaid S pSIug-siRNA and pNeg-SiRNA(negative control)were constructed and transfected into AsPC-1 cells.The expression of Slug was detected by RT-PCR and Western blotting.The proliferation of CaPan-2 cells in vitro was determined by MTT assay.The apoptotic rates of the cells were detected by flow cytometry.Results:Two cell clones were screened... |
| |
| Keywords: | Slug AsPC-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
