白细胞介素-1β及肿瘤坏死因子-α对脑损伤后胶质细胞c-fos mRNA表达的影响

本研究用分子杂交技术观察了白细胞介素-1β及肿瘤坏死因子-α对脑损伤后及培养的胶质细胞c-fosmRNA表达的影响。结果发现：脑损伤后损伤周围组织c-fosmRNA表达圣动态变化：1h后增加170％．12h后增加30％，24h后增加130％。发现白细胞介素-1β和肿瘤坏死因子-α能显著增加脑损伤后12h及24hc-fosmRNA的表达；当培养的胶质细胞中加入此二者后1hc-fosmRNA表达明显增加，12h后有所下降。又发现同时加入此二者时，c-fosRNA表达增加更加显著。以上结果提示；白细胞介素-1β和肿瘤坏死因子-α能影响脑损伤后胶质细胞c－fosmRNA的表达，它们对胶质细胞的作用可能与c-fos基因有关。

THE EFFECTS OF INTERLEUKIN-1β AND TUMOR NECROSIS FACTOR-a ON c-fos mRNA EXPRESSION OF GLIA AFTER BRAIN INJURY

The effects of interleukin-1β(IL1β) and tumor necrosis factor-α(TNF-α) on c-fos mRNA expression of injured brain and cultured glia were observed by dot blot hybridi;attion technique. The results showed that the c-fos rnRNA expres-sion of tissue around wound increased 170%, 30% and 130% at 1 h, 12 h and 24 h respectively after brain injury- IL1β and TNP-α signifitantly increased c-fos mRNA expression of tissue around wound at 12 h and 24 h affter brain injury. When pri-mary culture glia was treated with IL1β and TNF-α, the c-fos mRNA expression was enhanced significantly at 1 h and 12 h as compared with control group. But at 12 h the c-fos mRNA expression decreased when compared with 1 h group. When primary culture glia was treated with IL1β and TNF-a together, the expression of c-fos mRNA was more powerful compared with IL1β or TNF-α group. The results suggested that TNF-α and IL1β could influence glia c-fos mRNA expression after btain injury, and that their effects on glia may be associated with c-fos gene.