GP73与AFP-L3联合检测在原发性肝癌诊断中的临床价值

目的：探讨高尔基体蛋白73（GP73）和甲胎蛋白异质体3（AFP-L3）联合检测在原发性肝癌诊断中的临床价值。方法：采用免疫组化法和酶联免疫法检测原发性肝癌组（PHC组,n=45例）、肝硬化组（LC组,n=35例）和病毒性肝炎组（VH组,n=36例）患者肝组织和血清GP73、AFP-L3和AFP的表达水平,选择同期40例门诊正常体检者作为对照组。观察各组肝组织和血清GP73、AFP-L3和AFP的表达水平,并比较GP73、AFP-L3、AFP单一检测和GP73联合AFP-L3检测原发性肝癌的阳性率。结果：PHC组肝组织和血清GP73、AFP-L3和AFP表达水平明显高于LC组和VH组（P〈0.05）;PHC组AFP-L3和GP73单项检测阳性率（分别为68.9%、77.8%）均明显高于AFP检测阳性率（62.2%）（P〈0.05）,两指标联合血清检测可提高阳性率（86.7%）（P〈0.05）。结论：GP73和AFP-L3联合血清检测可提高原发性肝癌诊断阳性率,对原发性肝癌的早期诊断具有重要的临床意义。

Clinical value of GP73 combined detection with AFP-L3 in the diagnosis of primary hepatic carcinoma

Objective： To investigate the clinical value of Golgi protein 73 （ GP73 ） combined detection with alpha fetoprotein heterogeneity 3 （ AFP- L3 ） in the diagnosis of primary hepatic carcinoma. Methods： The expression levels of GP73, AFP- L3 and AFP in liver tissue and serum of primary hepatic carcinoma （ group PHC, n = 45 ）, liver cirrhosis （group LC, n = 35） and viral hepatitis （group VH, n = 36） patients were detected by immunohistochemical and enzyme immunoassay. At the same period, 40 cases of normal examination were selected as control group. The expression levels of GP73, AFP-L3 and AFP in liver tissue and serum of all groups were observed, and compared the positive rate of GP73, AFP-L3, AFP single detection and GP73 combined detection with AFP-L3 to primary hepatic carcinoma. Results： The expression levels of GP73, AFP-L3 and AFP in the liver tissue and serum of PHC group were significantly higher than those of LC group and VH group （ P 〈 0. 05 ）. The positive rate of AFP-L3 and GP73 in single serum detection （68.9%, 77.8% ） was obviously higher than that of AFP（62.2% ） in PHC group （ P 〈 0.05 ）, but lower than that of AFP- L3 combined serum detection with GP73 （ 86.7 % ） （ P 〈 0. 05 ）. Conclusion： The combined serum detection of GP73 and AFP- L3 can improve the positive rate of primary hepatic carcinoma. It has an important clinical significance on the early diagnosis of primary hepatocellular carcinoma.