| 复方止咳颗粒中甘草酸含量测定方法学研究 |
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| 引用本文： | 王玉巧,王健斌,赵一玫,刘英.复方止咳颗粒中甘草酸含量测定方法学研究[J].中国药事,2011,25(2):169-170,194. |
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| 作者姓名： | 王玉巧 王健斌 赵一玫 刘英 |
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| 作者单位： | 武汉红桃开国家企业技术中心,武汉,430074 |
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| 摘 要： | 目的采用RP-HPLC法对复方止咳颗粒中甘草酸的含量进行测定。方法采用Kromasil C18柱,流动相:甲醇-1%醋酸溶液(68∶32),流速:1.0mL.min-1,检测波长:250nm。结果甘草酸在0.17～4.25μg范围内有良好的线性关系,r=0.9999,平均回收率为98.4%,RSD为0.94%。结论本法简便快捷,结果准确,重复性好。
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| 关 键 词： | 复方止咳颗粒 甘草酸 RP-HPLC 含量测定 |
Study on Determination of Glycyrrhizic Acid in Zhike Complex Granules |
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| Wang Yuqiao,Wang Jianbin,Zhao Yimei,Liu Ying.Study on Determination of Glycyrrhizic Acid in Zhike Complex Granules[J].Chinese Pharmaceutical Affairs,2011,25(2):169-170,194. |
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| Authors: | Wang Yuqiao Wang Jianbin Zhao Yimei Liu Ying |
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| Institution: | Wang Yuqiao,Wang Jianbin,Zhao Yimei and Liu Ying(State-level Enterprise Technology Center of Wuhan RHK,Wuhan 430074) |
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| Abstract: | Objective A HPLC method was established for determination of glycyrrhizic acid in Zhike Complex Granules by RP-HPLC.Methods A Kromasil C18 column was used with methanol and 1% acetic acid（68∶32）as the mobile phase at the flow rate of 1.0mL·min-1.The detection wavelength was set at 250nm.Results The calibration range of glycyrrhizic acid was 0.17～4.25μg（r=0.9999）.The mean recovery was 98.4% and RSD was 0.94%.Conclusion The method is simple,rapid,accurate and reproducible. |
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| Keywords: | RP-HPLC |
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