| HPLC法测定牛大力中高丽槐素的含量 |
| |
| 引用本文： | 李锐,陈国彪. HPLC法测定牛大力中高丽槐素的含量[J]. 中国药事, 2011, 25(2): 124-125,140 |
| |
| 作者姓名： | 李锐 陈国彪 |
| |
| 作者单位： | 海南省药品检验所,海口,570216 |
| |
| 摘 要： | 目的建立HPLC法测定牛大力中高丽槐素含量的方法。方法采用依利特YWG C18色谱柱,流动相为乙腈-0.1%醋酸水溶液（40∶60）,检测波长为310nm,流速为1.0mL.min-1,柱温为27℃。结果高丽槐素进样量在0.06047～0.9675μg范围内线性关系良好（r=0.9999）;平均回收率为99.5%,RSD为1.80%（n=9）。结论该方法可用于牛大力中高丽槐素的含量测定。
|
| 关 键 词： | 牛大力 高丽槐素 HPLC |
Determination of Maackiain in Root of Millettia Speciosa by HPLC |
| |
| Li Rui,Chen Guobiao. Determination of Maackiain in Root of Millettia Speciosa by HPLC[J]. Chinese Pharmaceutical Affairs, 2011, 25(2): 124-125,140 |
| |
| Authors: | Li Rui Chen Guobiao |
| |
| Affiliation: | Li Rui and Chen Guobiao(Hainan Provincial Institute for Drug Control,Haikou 570216) |
| |
| Abstract: | Objective To establish a HPLC method for determination of Maackiain in root of Millettia speciosa.Methods YWG C18 column was used as chromatographic column.The mobile phase was acetonitrile-0.1% acetic acid solution（40∶60）.The flow rate was 1.0mL · min-1 and the detection wavelength was set at 310nm.The column temperature was maintained at 27℃.Results The calibration curve of Maackiain was in good linearity over the range of 0.06047～0.9675μg（r=0.9999）.The average recovery was 99.5%,with RSD of 1.80%（n=9）.Conclusion The method is suitable for determination of Maackiain in root of Millettia speciosa. |
| |
| Keywords: | HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
