加味生脉散对大鼠缺血再灌注肾脏损伤的保护作用

目的：观察加味生脉散对大鼠缺血再灌注肾脏损伤的保护作用。方法：双侧肾动脉夹闭45min，再灌注24h，造成大鼠缺血再灌注肾脏损伤模型。分别测定加味生脉散治疗组、病理模型组、假手术组大鼠血尿素氮（BUN）、血肌酐（Cr）、组织中髓过氧物酶（MPO）和丙二醛（MDA）浓度，并进行组织病理学检查。结果：与病理模型组相比，加味生脉散治疗组的缺血再灌注大鼠血尿素氮下降42．8％（P=0．024），血清肌酐下降55．9％（P=0．011）；肾脏组织MPO及MDA含量明显降低（P＜0．01）；组织病理学改变明显减轻（P＜0．05）。结论：加味生脉散可减轻缺血再灌注引起的肾脏损伤，其机制与降低缺血再灌注有关的自由基损伤和炎性损伤有关。

Protective effect of Jia-wei Sheng-mai-san on renal ischemia-reperfusion injury in rats

Objective: To reveal the protective effect of Ji a-wei Sheng-mai-san on renal ischemia-reperfusion injury in rats. Methods: Eighteen rats were divided randomly into three groups: group 1 ,sham operation; group 2, water (vehicle)-treated and group 3, Jia-wei Sheng- mai-san-treated. The rats in group 2 and group 3 were subjected to 45 min of w arm (renal) ischemia and then received reperfusion for 24 h. At the same time, the rats in group 1 received sham operation. Three 3 days before renal ischemia operation, Jia-wei Sheng-mai-san was administrated to rats of group 3.Results:Rats (administrated) with Jia-wei Sheng-mai-san ha d less functional impairment of the kidneys than those in vehicle-treated group . The histological changes were not as severe as those of controls.Compared with vehicle-treated group, increases in serum (creatinine) and blood urea nitrog en 24 h after reperfusion were attenuated by 55.9% (P=0.011) and by 42.8% ( (P=0.024)), respectively in group 3. The ischemia-reperfusion-induced in creases in tissue myeloperoxidase(MPO)and malondialdehyde(MDA) were reduced by 68% (P< 0.001) and 59%( P< 0.001) respectively in group 3.Tubular ce ll necrosis in medulla was less pronounced (P< 0.05) in animals administrate d with Jia-wei Sheng-mai-san. Conclusion: Jia-wei Sheng-m ai-san could partly mitigate renal damage or failure after ischemia-reperfusio n injury of the kidney by inhibition of neutrophil infiltration and oxidation in jury.