Use of random amplified polymorphic DNA-polymerase chain reaction for the definition of genetic markers for species and strains of porcine Oesophagostomum |
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| Authors: | A Joachim A Daugschies C M Christensen H Bjørn P Nansen |
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| Institution: | Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark, DK
Institut für Parasitologie, Tier?rztliche Hochschule Hannover, Bünteweg 17, D-30559 Hannover, Germany Fax: +49-511-9 53 88 70; e-mail: bruttkow@parasit.tiho-hannover.de, DE
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| Abstract: | Nodular worms are common parasites of pigs, and research has recently started to focus on the biology of these nematodes.
However, the methods for delineation of species at immature developmental stages and␣for␣differentiation of various lines
of the same species␣remain limited. For differentiation of porcine Oesophagostomum species and strains by genomic fingerprinting, random amplified polymorphic DNA-polymerase chain reaction was performed on
DNA derived from 20 larval batches of anthelmintic-susceptible and resistant strains and isolates of these nematodes and 2
ruminant Oesophagostomum spp. Polymorphic DNA markers could be amplified with 9 of the 33 primers tested. In all, 13 markers were species-specific
and 6 markers could differentiate between strains or groups of strains. With a combination of the latter, artificially selected
anthelmintic-resistant strains and the susceptible mother strain of O. dentatum could be delineated. When single adult worms were compared, considerable variations between strains of the same species and
between individuals from the same strain could be detected. The differentiation of Oesophagostomum strains and species at all parasitic stages on the basis of genetic markers could greatly facilitate studies on the biology
of these parasites.
Received: 6 January 1997 / Accepted: 24 March 1997 |
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