紫杉醇膀胱灌注抑制大鼠膀胱癌生长的机制

目的探讨紫杉醇连续膀胱灌注对大鼠膀胱肿瘤生长的作用和机制。方法将40只雌性Wistar大鼠,随机分为对照组(n=20)和紫杉醇组(n=20),制作N-甲基亚硝基脲诱导大鼠原位膀胱癌模型,于第8周分别行生理盐水和紫杉醇制剂连续膀胱灌注,每周1次,共6次。于第14周进行病理观察、测量膀胱总重量,微血管密度(MVD)和凋亡指数(AI)。结果第14周时,对照组(n=16)和紫杉醇组(n=12)大鼠膀胱均可见明显肿物,光镜下可见癌组织侵入肌层。紫杉醇组大鼠膀胱的重量和肿瘤的MVD小于对照组(P<0.01);紫杉醇组大鼠膀胱肿瘤的AI大于对照组(P<0.01)。结论紫杉醇连续膀胱灌注可抑制大鼠膀胱肿瘤生长,可能与抑制肿瘤血管生长和诱导肿瘤细胞凋亡有关。

The depressant effects and mechanism of intravesical instillation of paclitaxel in rats with bladder cancer

Objective: To investigate the effects and mechanism of intravesical instillation of paclitaxel in orthotopic bladder tumor of rats. Methods: Forty female Wistar rats were randomized into control group (n = 20) and paclitaxel group (n =20). Orthotopic bladder tumors were established in rats by intravesical administration of N-mmethyl-noitrosourea. Rats of control and paclitaxel group received a total of six times (every week) intravesical instillation of physiologic saline and paclitaxel respectively from the eighth week. Rats were executed in the fourteenth week and their weights of bladder, pathologic characters, microvessel densities (MVD), apoptotic indexes (AI) were surveyed. Results: There were obvious neoplasms in both control group (n=16) and paclitaxel group (n=12) in the fourteenth week. The cells of bladder carcinoma had developed in muscular layes of bladder. The bladder weights and MVDs of paclitaxel group were significantly lower than those in control group (P<0.01), but the AI of control group were significantly lower than those in paclitaxel group (P<0.01). Conclusion: Intravesical instillation of paclitaxel can inhibit the growth of bladder tumour by restraining angiogenesis and accelerating apoptosis in rats.