前S1抗原、HBV DNA及HBeAg在乙型肝炎病毒检测中的临床意义

目的 了解乙型肝炎患者前S1抗原(Pre-S1)、HBV DNA及HBeAg的相关性,并评价它们在诊断HBV复制中的临床意义.方法 应用ELISA、荧光定量PCR法(FQ-PCR)对198例乙型肝炎患者进行Pre-S1、HBV DNA及HBV血清标志物五项(两对半)检测.结果 198例乙型肝炎患者中Pre-S1阳性107例(54.0%),HBV DNA阳性111例(56.1%),HBeAg阳性90例(45.5%),三者同时阳性72例(36.4%),Pre-S1与HBV DNA阳性率比较,差异无统计学意义(P>0.05).在90例HBeAg阳性样本中,Pre-S1阳性72例(80.0%),HBVDNA阳性74例(82.2%),三者具有较高的符合率.在108例HBeAg阴性样本中,Pre-S1阳性35例(32.4%),HBV DNA阳性37例(34.2%),Pre-S1与HBV DNA检出率比较,两者高度一致(χ2=0.08,P>0.05).结论 Pre-S1与HBV DNA能敏感反映HBV复制的情况,尤其可以协助诊断HBeAg阴性的乙型肝炎患者是否有HBV复制,对于判断患者的病情及指导治疗具有重要的临床意义.

Clinical significance of Pre-S1 antigen, HBV DNA, HBeAg in the detection of hepatitis B virus

Objective To study the associations among Pre-S1 antigen(Pre-S1), HBV DNA and HBeAg and explore the significance of HBV Pre-S1, HBV DNA, HBeAg detection in the viral replication. Method Pre-S1, serum markers of HBV and HBV DNA concentrations of 198 sere of hepatitis B patients were de,ted by ELISA and FQ-PCR. Results The positive rates of Pre-S1, HBV DNA and HBeAg were 54.0% (107/198), 56.1% (111/198) and 45.5%(90/198) respectively among 198 patients . The same positive rates of them were 36.4%(72/198). In 90 patients with HBeAg positive, the positive rates of Pre-S1 and HBV DNA were 80% (72/90) ,82.2% (74/171) respectively,the coincidence rates of them were great ( P > 0.05). In 108 patients with HBeAg negative, the positive rates of Pre-S1 and HBV DNA were 32.4% (35/108),34.2% (37/108) respectively. There was no significant difference in positive rate between Pre-S1 and HBV DNA( χ2 = 0.08, P > 0.05). Conclusions Pre-S1 and HBV DNA can reflect the replication of HBV sensitively, especially for those who are HBeAg negative, and can be used as a helpful tool for the diagnosis of HBV infection and clinical evaluation.