| 高效液相-电喷雾质谱分析7-羟基黄酮在大鼠体内的代谢产物 |
| |
| 引用本文： | 韦英杰,姜金生,谭晓斌,陈斌,薛小璐,胡春萍,马世平,贾晓斌. 高效液相-电喷雾质谱分析7-羟基黄酮在大鼠体内的代谢产物[J]. 中国医院药学杂志, 2012, 0(4): 241-243 |
| |
| 作者姓名： | 韦英杰 姜金生 谭晓斌 陈斌 薛小璐 胡春萍 马世平 贾晓斌 |
| |
| 作者单位： | 江苏省中医药研究院中药新型给药系统重点实验室国家中医药管理局中药口服释药系统重点研究室;中国药科大学中药药理教研室 |
| |
| 基金项目： | 国家自然科学基金(编号:30973978) |
| |
| 摘 要： | 目的:研究7-羟基黄酮在大鼠体内的代谢。方法:应用高效液相-电喷雾质谱检测大鼠灌胃7-羟基黄酮后血浆、尿液、胆汁和粪便中的代谢产物。实验采用Zorbax C18色谱柱,二元线性梯度洗脱进行色谱分离,并与电喷雾质谱联用,根据负离子模式的分子离子峰获得化合物相对分子质量信息,推测化合物的可能结构。结果:在大鼠尿液、粪便、血浆、胆汁中检测到原形成分7-羟基黄酮和7-羟基黄酮葡萄糖醛酸结合物,在胆汁或尿中尚检测到7-羟基黄酮硫酸结合物。结论:7-羟基黄酮在大鼠体内主要以Ⅱ相代谢产物葡萄糖醛酸结合物和硫酸结合物的形式存在。
|
| 关 键 词： | 7-羟基黄酮 代谢 大鼠 高效液相-电喷雾质谱 |
Analysis on the metabolites of 7-hydroxyflavone in rats by high performance liquid chromatography-electrospray mass spectrometry |
| |
| WEI Ying-jie,JIANG Jin-sheng,TAN Xiao-bin,CHEN Bin,XUE Xiao-lu,HU Chun-ping,MA Shi-ping,JIA Xiao-bin. Analysis on the metabolites of 7-hydroxyflavone in rats by high performance liquid chromatography-electrospray mass spectrometry[J]. Chinese Journal of Hospital Pharmacy, 2012, 0(4): 241-243 |
| |
| Authors: | WEI Ying-jie JIANG Jin-sheng TAN Xiao-bin CHEN Bin XUE Xiao-lu HU Chun-ping MA Shi-ping JIA Xiao-bin |
| |
| Affiliation: | 1(1.Key Laboratory of New Drug Delivery System of Chinese Meteria Medica,Jiangsu Provincial Academy of Chinese Medicine,Jiangsu Nanjing 210028,China;2.Department of Pharmacology of Chinese Materia Medica,China Pharmaceutical University,Jiangsu Nanjing 210009,China) |
| |
| Abstract: | OBJECTIVE To study the metabolism of 7-hydroxyflavone in rats.METHODS High performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS) was used for the analysis of metabolites of 7-hydroxyflavone in rats after oral administration.The separation was performed with a Zorbax C18 column using a binary gradient elution of 0.05% formic acetonitrile-0.05% formic acid water.The quasi-molecular ions of compounds in positive mode were observed for molecule mass information,and the potential structures were presumed.RESULTS Parent component 7-hydroxyflavone and glucuronide conjugate of 7-hydroxyflavone were found in rats’ urine,faece,plasma and bile,and sulfate conjugate of 7-hydroxyflavone was found in urine or bile.CONCLUSION Glucuronide conjugate of 7-hydroxyflavone and sulfate conjugate of 7-hydroxyflavone were the major phase Ⅱ metabolites in rats. |
| |
| Keywords: | 7-hydroxyflavone metabolism rat high performance liquid chromatography-electrospray mass spectrometry |
| 本文献已被 CNKI 等数据库收录！ |
|
