泄浊除痹方总黄酮对小鼠肾小管上皮细胞增殖与尿酸吸收的影响

目的:研究泄浊除痹方总黄酮对小鼠肾小管上皮细胞(RTECs)增殖与尿酸吸收的影响。方法:采用优化后的小鼠RTECs原代培养方法分离该细胞,不同浓度的泄浊除痹方总黄酮孵育48h,MTT法检测细胞活性,配制尿酸吸收培养基,进行尿酸吸收实验,采用尿酸检测试剂盒测定尿酸吸收值。结果:所分离培养的细胞为目的细胞,即小鼠RTECs,具有尿酸吸收功能;高浓度药物(10、7.5、5.0g.L-1)对细胞增殖有不同程度的抑制作用,低浓度药物(2.5、0.5g.L-1)对细胞增殖无显著性影响;在对细胞生长活性无显著性影响的浓度梯度范围内(2.5、2.0、1.5、1.0、0.5g.L-1),药物对尿酸吸收表现出不同程度的抑制作用。结论:泄浊除痹方总黄酮对小鼠RTECs尿酸吸收功能有一定的抑制作用。

Effects of Total Flavonoids in Xiezhuo Chubi Decoction on the Proliferation and Uric Uptake Function of Mice Renal Tubular Epithelial Cells

OBJECTIVE:To investigate the effects of total flavonoids in Xiezhuo chubi decoction on the proliferation and uric uptake function of mice renal tubular epithelial cells(RTECs) in vivo.METHODS:The RTECs were isolated from the renal tubular epithelial cells of mice by optimized primary culture method,and were cultured with total flavonoids from Xiezhuo chubi decoction of different concentrations.The activity of RTECs was assayed by MTT assay.The uric uptake medium was prepared and the uric uptake values were determined by the kit.RESULTS:The interest cells were isolated,i.e.mice renal tubular epithelial cells,and it had the function of uric uptake.The proliferation of the RTECs was inhibited by high concentrations(10,7.5,5.0 g·L-1) of the drug.No significant effects on the proliferation of the RTECs were observed in the low concentrations(2.5,0.5 g·L-1);total flavonoids had no significant effect on growth activity of cells in this range of 2.5,2.0,1.5,1.0,0.5 g·L-1.total flavonoids inhibited uric uptake in different degree.CONCLUSION:The uric uptake function of the RETCs can be inhibited by the total flavonoids in Xiezhuo chubi decoction.