| 人体铜伴侣蛋白Atox1在大肠杆菌中的表达 |
| |
| 引用本文： | 张雪峰,高旭,尹王申,周虹.人体铜伴侣蛋白Atox1在大肠杆菌中的表达[J].基础医学与临床,2001,21(5):471-473. |
| |
| 作者姓名： | 张雪峰 高旭 尹王申 周虹 |
| |
| 作者单位： | 哈尔滨医科大学生物化学教研室 |
| |
| 摘 要： | 利用PCR技术扩增出人体转铜伴侣蛋白Atox1和cDNA片段，直接克隆到PCRⅡ载体上，经DNA序列测定后，再插入到谷胱甘肽巯基转移酶（GST）融合表达载体PGEX－6p-2上，构成重组表达质粒PGEA，将此质粒导入大肠杆菌，经IPTG诱导后获得PGEA融合蛋白的表达。表达的融合蛋白经亲和层析、位点特异性蛋白酶切得到纯化的Atox1蛋白。
|
| 关 键 词： | 转铜伴侣 基因重组 亲和层析 融合蛋白 大肠杆菌 表达 |
| 文章编号： | 1001-6325(2001)05-0471-03 |
| 修稿时间： | 2000年8月31日 |
Expression of human Copper Chaperone Protein Atox1 in E.coli |
| |
| ZHANG Xue feng,GAO Xu,YIN Shen,et al.Expression of human Copper Chaperone Protein Atox1 in E.coli[J].Basic Medical Sciences and Clinics,2001,21(5):471-473. |
| |
| Authors: | ZHANG Xue feng GAO Xu YIN Shen |
| |
| Abstract: | cDNA of human copper chaperone Atox1 was amplified by PCR and cloned into PCRII vector After DNA sequencing, it was cloned into GST fusion protein expressive vector PGEX 6p 2. The recombinant plasmid PGEA was constructed and introduced into E coli GST Atox1 fusion protein was expressed at the induction of IPTG and was purified by affinity chromatography By cleavage of site specific protease and second affinity chromatography, GST was removed and recombinant Atox1 protein was purified |
| |
| Keywords: | copper chaperone genetic recombination affinity chromatography fusion protein |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
