浸润性T淋巴细胞及Th细胞因子在不同类型乳腺癌中的表达

目的研究浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达的意义。方法利用免疫组织化学链霉亲和素生物素法（labelled streptavidin-biotin method，LSAB）方法检测61例浸润性微乳头状癌（invasive micropapillary carcinoma，IMPC）、71例浸润性导管癌（invasive ductal carcinoma，IDC）和27例髓样癌（medullary carcinoma，MC）3种乳腺癌中浸润T淋巴细胞亚群分布以及Th细胞因子的表达。T淋巴细胞组间比较采用Kruskal-Wallis Test，组内比较采用Witcoxon Signed Ranks Test；组间Th细胞因子的比较采用x^2检验。结果CD3、CD8和CD4均表达于淋巴细胞胞膜。CD3^＋T细胞在3组间差异无统计学意义（P=0．735）。CD8^＋T和CD4^＋T细胞在3组间差异均有统计学意义（P=0．000，P=0．012）。Th细胞因子主要表达于肿瘤细胞和淋巴细胞的胞质。IL-2表达在IMPC组最低（25／61，41．0％），其次是IDC组（33／71，46．5％），最高是MC组（20／27，74．1％），3组间差异有统计学意义（P=0．014）；IFN-γ的表达类似于IL-2，IMPC组最低（39／61，63．9％），其次是IDC组（57／71，80．3％），最高是MC组（24／27，88．9％），3组间差异有统计学意义（P=0．019）；IL-4的表达在IDC组最高（42／71，59．2％），其次是IMPC组（19／61，31．1％），最低是MC组（5／27，18．5％），3组间差异有统计学意义（P=0．000）；IL-10的阳性率在IMPC组是87．8％（47／61），在IDC组是87．3％（62／71），在MC组是88．9％（24／27），3组间差异无统计学意义（P〉0．050）。结论浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达不同。

Expression of infiltrating T lymphocytes and T helper cytokines in different types of breast cancer

Objective To study the expression of tumor-infiltrating lymphocytes （TILs） and T helper cytokines in invasive micropapillary carcinoma （IMPC）, invasive ductal carcinoma （IDC） and medullary carcinoma （MC） of the breast. Methods Three T-cell markers including pan-T cell marker （CD3）, T-helper cell marker （CD4）, cytotoxie T cell marker （CD8）, and four T-helper cytokines including interleukin-2 （IL-2）, interferongamma （IFN-γ）, interleukin-4 （IL-4）, and interleukin-10 （IL-10） were tested immunohistochemically in the three groups, （IMPC n=61, IDC n=71, and MC n=27） on formalin-fixed, paraffin embedded tumors. Kruskal-Wallis Test and Wilcoxon Signed Ranks Test were used for analysis of TILs;chi-square test was used for analysis of T helper cytokines. Results Markers, CD3, CD4 and CDS, all expressed in the cell membrane of lymphocytes. There was no statistical difference in CD3＋T cells number between the three groups （P= 0. 705）; CD8^＋ T cells and CD4^＋ T cells were statistically different between the three groups （P=0. 000,P=0. 012）. T helper cytokines expressed in cytoplasm of tumour cells and lymphocytes. The expression of IL-2 was the highest in the MC group （20/27,74. 1%）, followed by the IDC group （33/71,46. 5%）, and the lowest in the IMPC group （25/61, 41.0%）, there was a statistical difference between the three groups（P =0. 014）. The expression of IFN-γ was the highest in the MC group （24/27,88. 9%） followed by the IDC group（57/71,80. 3%）, and the lowest in the IMPC group（39/61,63.9%）, there was a statistical difference between the three groups（P = 0. 019）. The positive expression of IL-4 was the highest in the IDC group （42/71,59. 2%）, followed by the IMPC group （19/61, 31.1%） and the lowest in the MC group （5/27, 18. 5%）, there was a statistical difference between the three groups （P=0. 000）. The positive rate of IL-10 expression was 87.8%（47/61）in the IMPC group, 87.3%（62/71） in the IDC group