High Bilirubin Levels Interfere with Serum Tartrate-Resistant Acid Phosphatase Determination: Relevance as a Marker of Bone Resorption in Jaundiced Patients |
| |
Authors: | L Alvarez P Peris J L Bedini A Parés A Monegal N Guañabens E Mas C Aibar A M Ballesta |
| |
Institution: | (1) Service of Biochemistry, Hospital Clinic, University of Barcelona, C/Villarroel 170, Barcelona 08036, Spain, ES;(2) Service of Rheumatology, Hospital Clinic, University of Barcelona, C/Villarroel 170, Barcelona 08036, Spain, ES;(3) Metabolic Bone Diseases Unit, Hospital Clinic, University of Barcelona, C/Villarroel 170, Barcelona 08036, Spain, ES;(4) Liver Unit, Hospital Clinic, University of Barcelona, C/Villarroel 170, Barcelona 08036, Spain, ES |
| |
Abstract: | Serum tartrate-resistant acid phosphatase (TRAcP) activity is considered to be a biochemical marker of bone resorption. Recently,
a lack of specificity of collagen-related markers for assessing bone turnover has been observed in patients with chronic liver
disease. Thus, it could be of great interest to determine serum TRAcP activity in such patients. However, nonspecificity of
the analytical reaction could occur when hemolyzed, lipemic, or icteric specimens are analyzed. Therefore, we have studied
the interference caused by bilirubin in the measurement of serum TRAcP activity using the Hillmann method. The interference
was assessed in two pools of serum containing different bilirubin concentrations but with similar total AcP levels. Mixing
proportional parts of the two pools, 10 samples were also obtained. Serum activities of total AcP and TRAcP, and the concentration
of bilirubin were measured in the 10 samples. Both the actual and the expected values obtained by theoretical calculations
were compared. Serum bilirubin values of 2.4 mg/dl showed a negative interference of 15% in the determination of serum TRAcP
activity, whereas values of bilirubin higher than 10 mg/dl interfered totally with the measurement of serum TRAcP. Bilirubin
did not interfere with the total AcP determination. This study clearly shows the interference of bilirubin in the determination
of serum TRAcP. This finding should be considered when bone metabolism disorders are evaluated in jaundiced patients.
Received: 6 April 1998 / Accepted: 1 October 1998 |
| |
Keywords: | : Hepatic diseases — Biochemical markers — Bone turnover — Acid phosphatase |
本文献已被 PubMed SpringerLink 等数据库收录! |
|