系统性红斑狼疮患者自身抗体检测及意义

目的：探讨自身抗体在系统性红斑狼疮（SLE）中的意义。方法：采用放射免疫分析检测50例SLE患者血清中的抗双链DNA（dsDNA）抗体，采用德国IMTEC公司抗核抗体谱线性印迹法检测抗核小体抗体（AnuA）、抗组蛋白抗体（AHA）、抗StuD1等其他自身抗体。结果：抗StuD1阳性率最高（82％），其次是抗R060KD抗体（80％）和AunA（72％）；抗dsDNA、AHA、抗U1-RNP、抗R052KD、抗SSB的阳性率分别为44％、32％、58％、48％、24％。其他三种自身抗体阳性率很低。结论：SLE患者血清中可出现大量的自身抗体，同时检测多种自身抗体能提高对SLE的鉴别诊断。

Significance of Combined Determination of Multiple Autoantibodies in Patients with Systemic Lupus Erythematosus

Objective To explore the roles played by autoantibodies in systemic lupus erythematosus.Methods Serum anti-dsDNA antibody(with RIA) and serum anti-nucleosome antibody(AnuA),AHA,anti SmD1-,anti Ro60UD-,anti U1-RNP-,anti-Ro52KD,anti-SSB-antibodies(with anti-nucleo antibodies linear spectrum blotting method) were detected in 50 patients with clinically proven systemic lupus erythematosus.Results The positive rate with anti-SmD1 antibody was highest(82%),followed by anti-Ro60KD antibody(80%) and AnuA(72%).Positive rate with anti dsDNA-,AHA,anti-U1-RNP-,anti-Ro52KD and anti SSB-antibodies was 44%,32%,58%,48% and 24% respectively.Positive rate with anti-SC1-70,ACA and Jo-1 antibodies was extremely low(below 10%).Conclusion Multiple auto-antibodies were present in serum of patients with systemic lupus erythematosus and combined detection of them would improve the diagnostic sensitivity.