P16MTS1、P27KIP1基因在胆管癌组织中的表达研究

目的：探讨P16^MIS1,P27^KIP1基因与胆管癌临床病理的关系。方法：采用免疫组化方法，检测48例胆管癌和8例伴慢性炎症的胆管壁组织中P16^MIS1,P27^KIP1蛋白表达情况。结果：（1）胆管癌中P16^MIS1,P27^KIP1阳性表达率分别为47．9％和35．4％，而伴炎症的胆管组织阳性表达率为100％和87．5％；（2）P16^MIS1,P27KIP1蛋白表达与胆管癌的分化程度，有无淋巴结或远处转移以及临床分期呈显著相关性。结论：P16^MIS1,P27^KIP1多基因表达异常可能与胆管癌的发生发展有关，是衡量胆管癌预后的有用指标。

Expressions of P16MTS1 and P27KIP1 in cholangiocarcinoma

Objective To study the P16 MTS1 and P27 KIP1 expressions in human cholangiocarcinoma tissues and their relationship with clinical pathology. Methods The expressions of P16 MTS1 and P27 KIP1 were examined in 48 samples of cholangiocarcinoma tissues with pathological confirmation and 8 specimens of bile duct with chronic inflammation were determined with immunohistochemical method. Results The expression rates of P16 MTS1 and P27 KIP1 were 47.9% and 35.4% respectively in cancerous tissues, but 100% and 87.5% in normal bile duct tissues with chronic inflammation. The expressions of P16 MTS1 and P27 KIP1 had a significantly correlation with differentiation, lymph node or distant metastases, and clinical stage of cholangiocardinoma. Conclusion Our results suggest that aberrant expressions of P16 MTS1 and P27 KIP1 may be associated with the incidence and development of cholangiocarcinoma and can be regarded as prognostic markers.