浙江省HIV-1流行毒株的基因序列测定和亚型分析

目的 利用脱氧核糖核酸(DNA)序列分析浙江省境内艾滋病病毒1型(HIV-1)流行毒株的亚型，为艾滋病预防控制工作提供参考。方法 采取14份浙江省HIV-1抗体阳性者的全血，提取前病毒DNA作为扩增模板，设计引物对enz，基因的C2-V3区进行聚合酶链反应(PCR)扩增并测定其序列。所得序列用Wisconsin公司的GCG软件包进行分析，确定亚型。结果 14份标本中有9份扩增阳性并得到相应序列，扩增率为64．3％。经过离散率计算和系统树分析后证实：4份标本为E亚型的A／1三流行重组模式，3份标本为B亚型，1份标本为C亚型的B／C流行重组模式，1份标本为G亚型。结论 目前浙江省境内有多种亚型的HIV-1毒株存在，流行形势严峻。迫切需要深入调查研究，并在高危人群中开展行为干预措施。

Subtype and sequence analysis of the C2-V3 region of gp120 genes among HIV-1 strains in Zhejiang province

Objective To identify the HIV-1 subtype endemic in Zhejiang by nucleotide sequencing and to provide valuable information on prevention of AIDS for the government Methods In the study,DNA fragments of HIV-1 env gene were amplified by PCR from the whole blood obtained from 14 persons infected with HIV in the province The amplified products of C 2-V 3 region were analyzed by Wisconsin software package or Genetics Computer Group(GCG) Results Sequence analysis showed that there were four HIV-1 subtypes,E(4 samples),B(3 samples),C(1 sample)and G(1 sample)in the province Discussion Several subtypes exist in Zhejiang province and the epidemic situation of AIDS is serious More intensive investigations and behavioral interventions in high risk population groups should be developed