芦荟苦素对体外培养的黑素细胞影响的实验研究

目的：研究芦荟苦素对体外培养的正常人表皮黑素细胞的作用效应。方法：利用碱性成纤维细胞生长因子为主要有丝分裂原建立正常人表皮黑素细胞培养系，MTT法测定芦荟苦素对黑素细胞活力的影响，以左旋多巴为底物测定酪氨酸酶活性，图像信号采集与分析系统测定黑素含量，并与熊果苷及茶多酚的结果进行比较。结果：芦荟苦素对体外培养的正常人黑素细胞酪氨酸酶活性呈浓度依赖性抑制，可显著减少黑素生成量，对黑素细胞活力影响很小。结论：芦荟苦素对体外培养的人黑素细胞的酪氨酸酶活性及黑素生成有显著抑制作用，且细胞毒性很低，值得进一步研究。

Experimental study of effects of aloesin on cultured human melanocytes in vitro

Objective To study the effects of aloesin on normal cultured human melanocytes in vitro. Methods Basic fibroblast growth factor (bFGF) was employed to establish the culture of human melanocytes .The cultured melanocytes in vitro were treated with aloesin, the cell viability, tyrosinase activity was measured by MTT assay, utilization of L -Dopa as the substrate respectively; melanin content was measured by image analysis system. Furthermore, the effects of aloesin on melanocytes were compared with that of arbutin and tea polyphenols. Results Aloesin showed a concentration - dependment reduction in tyrosinase activity of human melanocytes. Melanin content was reduced significantly by aloesin, which has little influence on melanocytes viability. Conclusion Aloesin can significantly inhibit the tyrosinase activity and melanogenesis of cultured human melanocytes with low cytotoxicity. It is worth to study later.