“关元俞”、“膀胱俞”传入神经元与膀胱传入神经元的节段性分布及联系(经穴脏腑相关-足太阳膀胱经研究)

兔30只,分为三组。第一组10只,将10％HRP80微升注入“关元俞”,观察其传入神经元的节段分布;第二组10只,将10％HRP80微升注入“膀胱俞”,观察其传入神经元的节段分布;第三组10只,将10％HRP100微升注入膀胱壁内,观察其传入神经元的节段分布。“关元俞”穴位的传入神经元的节段为腰1－骶5脊神经节,“膀胱俞”穴位的传入神经元节段为腰2－骶5脊神经节,膀胱的传入神经元节段为胸12－尾1脊神经节。从上述结果可以证实,膀胱传入神经元节段与“关元俞’、“膀胱俞”两穴位传入神经元节段相互重叠9个节段,即腰2－骶5。这些重叠与交汇的形态学观察证实了当针灸这两穴位时传入神经元将刺激传至脊髓并可经上行束至丘脑进行整合,对膀胱机能进行调整以达到治疗的效果。这些实验可初步用神经解剖学方法阐明针刺“关元俞”、“膀胱俞”治疗泌尿系疾患的机理,也为经穴脏腑相关学说提供了形态学理论依据。

The Segmental Distribution and Connection of Afferent Neurons of Urinary Bladder, "Guan Yuan Shu", "Pang Guang Shu" Points in the Spinal Ganglion and Spinal Cord

Thirty rabbits, weighing 2. 5 - 3.0kg, were divided into three groups of 10 rabbits each. 10% HRP 50 - 80μl was injected into "Guan Yuan Shu" point and "Pang Guang Shu" point and 10% HRP 100 - 150μl injected into the urinary bladder. The animals were survived for 3 - 4 days, using 1% paraformaldehyde solution in phosphate buffer to perfuse their hearts, then take the thoracic ganglia,lombar ganglia and their spinal cord, which were made frozen sections of 40μm and TMB method reaction. The segmental distribution of afferent neurons of point and urinary bladder was observed under the light microscope.The segmental distribution of afferent neurons of the "GuanYuan Shu" point was L1-S5, the "Pang Guang Shu" Point was L2 - S5, urinary bladder was T12 - L1 - CO1. These afferent nerve fibers proected into Ⅲ- Ⅳ lamina.The experimental studies show that the afferent neurons of "Guan Yuan Shu", "Pang Guang Shu"POint and urinary bladder are mutual intersection and overlap in the spinal ganglia of L2 - S5, extending to 9 segments. These afferent fibers converge to the Ⅲ- Ⅳ lamina in spinal cord.The mutual intersection and convergence of these fibers may be the morphological foundation of regulating the function of urinary bladder with acupuncture at "Guan Yuan Shu", "Pang Guang Shu" point.The results may elucidate the mechanism of correlation between acupoint and viscera too.