新型无血预充大鼠体外循环模型的建立

目的建立简便易行的新型无血预充大鼠体外循环模型，为进行中长期研究体外循环对机体各器官的影响提供物质基础。方法选用7只成年SD大鼠，七氟烷诱导麻醉，经口气管插管接呼吸机维持麻醉。左侧股动脉22G套管针穿刺监测血压，鼠尾动脉置入20G套管针为动脉端灌注，经颈静脉置入自制多孔静脉引流管为静脉引流，转流90min后停机，监测术前、术中和术后各时间点血流动力学和血气数值。结果各时间点血流动力学和血气数值满意，7只大鼠停机后生命体征稳定。结论成功建立新型无血预充大鼠体外循环模型，麻醉深度和转流流量和温度均可精确控制，为进一步实施干预性、以及慢性长期实验提供可靠的模型基础。

Establishment of a novel rat model bloodless priming during normathermia cardiopulmonary bypass

Objective To establish simple and convenient cardiopulmonary bypass(CPB) model bloodless priming in rats.Methods Seven adult rats received sevoflurane inhalation anesthesia and maintained anesthesia state by tracheal intubation.During the preoperative preparation,22G catheter was cannulated in left femoral artery for monitoring arterial blood pressure,20G catheter was cannulated in tail artery for the arterial inflow,homemade multi-orificed catheter was cannulated in right jugular vein for venous drainage.All CPB process lasted for 90 minutes.Blood gas analysis and homodynamic parameters were measured in each time point before CPB,during CPB and after CPB.Results Blood gas analysis and hemodynamic parameters of each time points accorded with normal standard.Vital signs of all rats were stable.Conclusion The establishment of CPB bloodless priming in rats is successful.The model could provide reliable basic for further research by controlling the depth of anesthesia and temperature precisely.