| 高效液相色谱法测定人血清中胺碘酮浓度 |
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| 引用本文： | 季闽春,沈晓英,朱淑贤,杨耀芳.高效液相色谱法测定人血清中胺碘酮浓度[J].中国药房,2007,18(5):352-353. |
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| 作者姓名： | 季闽春 沈晓英 朱淑贤 杨耀芳 |
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| 作者单位： | 复旦大学附属上海市第五人民医院,上海市,200240 |
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| 摘 要： | 目的建立以高效液相色谱法测定人血清中胺碘酮浓度的方法。方法色谱柱为ShimadzuShim-packVP-ODS,流动相为甲醇∶乙腈∶醋酸-醋酸钠缓冲液(80∶10∶10),流速为0.8mL.min-1,紫外检测波长为241nm,内标为枸橼酸他莫昔芬。结果胺碘酮检测浓度在0.4～3.2mg·L-1范围内线性关系良好(r=0.9997),方法回收率为101.4%～109.0%,绝对回收率为79.5%～87.0%,日内和日间RSD<10%。结论本方法快速、专一,可用于人血清中胺碘酮浓度的测定。
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| 关 键 词： | 高效液相色谱法 胺碘酮 血药浓度 |
| 文章编号： | 1001-0408（2007）05-0352-02 |
| 收稿时间： | 2006-03-07 |
| 修稿时间： | 2006-08-03 |
Determination of Amiodarone in Human Serum by HPLC |
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| JI Minchun,SHEN Xiaoying,ZHU Shuxian,YANG Yaofang.Determination of Amiodarone in Human Serum by HPLC[J].China Pharmacy,2007,18(5):352-353. |
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| Authors: | JI Minchun SHEN Xiaoying ZHU Shuxian YANG Yaofang |
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| Abstract: | OBJECTIVE:To determine amiodarone in human serum by HPLC.METHODS:The separation was carried out on a Shimadzu Shim-pack VP-ODS column.The mobile phase consisted of methanol∶acetonitrile∶acetic acid-sodium acetate buffer(80∶10∶10) at a flow rate of 0.8mL·min-1.UV detector wavelength was 241nm.Tamoxifen citrate served as internal standard.RESULTS:The linear range of amiodarone was 0.4～3.2mg·L-1(r=0.999 7);The method recovery and the absolute recovery were 101.4%～109.0%,79.5%～87.0% respectively;the intra-day and inter-day RSD<10%.CONCLUSION:The method is rapid,specific and suitable for the determinatien of serum amiodarone. |
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| Keywords: | HPLC Amiodarone Blood concentration |
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