维生素E对LPS诱导的急性肺损伤中氧化应激的影响

目的:探讨维生素E对脂多糖(lipopolysaccharide,LPS)诱导的急性肺损伤(acute lung injury,ALI)中氧化应激状态的影响。方法:以Wistar大鼠为研究对象,实验分成正常对照组、ALI组及维生素E预处理组。用LPS作用于大鼠6 h后检测各组大鼠肺组织中丙二醛(Malondialdehyde,MDA)的含量、乳酸脱氢酶(lactate dehydrogenase,LDH)及超氧化物歧化酶(superoxide dismutase,SOD)活性,并应用RT-PCR及ELISA法检测大鼠肺组织中TNF-α的水平。结果:ALI组与正常对照组比较SOD活性下降、LDH的活性增强、MDA的含量升高,且TNF-αmRNA及蛋白的表达增加(P﹤0.05);维生素E预处理组与ALI组比较SOD活性增强,LDH的活性减弱,MDA的含量降低,TNF-αmRNA及蛋白的表达降低(P﹤0.05)。结论:维生素E预处理可减轻LPS诱导的ALI大鼠肺组织的脂质过氧化损伤,减少炎症因子的表达,发挥一定的抗损伤效应。

Effect of Vitamin E on oxidative stress of LPS-induced ALI

Objective To explore the effect of vitamin E on oxidative stress of lipopolysaccharide(LPS) induced acute lung injury(ALI).Methods Wistar rats as the research object,the experiment is divided into normal group,ALI group and vitamin E pretreatment group.After LPS treat rats on 6 hours,MDA content,LDH and SOD activity in the lung tissue were detected,RT-PCR and ELISA were used to detect TNF-α expression of lung.Results ALI groups activity of SOD decreased,activity of LDH strengthen,content of MDA increased and TNF alpha mRNA and protein expression increased compared with normal groups(P﹤0.05).Vitamin E pretreatment groups activity of SOD strengthen,activity of LDH abated,content of MDA reduced,and TNF alpha mRNA and protein expression reduced compared with LPS groups(P﹤0.05).Conclusion Vitamin E pretreatment can reduce lipid peroxidation damage and inflammatory factor expression of lung tissue in LPS-induced ALI,playing against-injury effect.