重组碱性成纤维细胞生长因子/I型胶原复合材料修复兔半月板无血运区软骨损伤

摘要背景：单独应用重组碱性成纤维细胞生长因子对无血运区半月板损伤的愈合无明显影响，考虑主要与其在体内易被吸收和降解等有关。近年来Ⅰ型胶原作为生物性载体的应用逐渐增多。目的：观察重组碱性成纤维细胞生长因子/Ⅰ型胶原复合材料修复兔半月板软骨损伤的效果。方法：选用健康新西兰大白兔36只，首先在兔半月板上造成统一的无血运区损伤模型。随机分为空白对照组、重组碱性成纤维细胞生长因子组和重组碱性成纤维细胞生长因子/Ⅰ型胶原组。术后2，6，12周分批处死实验动物，进行大体形态观察和组织学检查。结果与结论：重组碱性成纤维细胞生长因子组对无血运区损伤愈合无明显影响，但对半月板边缘滑膜中成纤维细胞有明显的促增殖作用；重组碱性成纤维细胞生长因子/Ⅰ型胶原组表现为纤维软骨样组织愈合，但其愈合组织与正常半月板组织仍有差异。结果表明应用重组碱性成纤维细胞生长因子/Ⅰ型胶原复合型材料治疗半月板无血运区的损伤是一种有效的治疗方法。关键词：半月板；软骨；损伤；重组成纤维细胞生长因子；I型胶原doi:10.3969/j.issn.1673-8225.2010.34.006

Recombinant basic fibroblast growth factor/type I collagen composite materials repair meniscal cartilage injuries in avascular zone of rabbits

AbstractBACKGROUND: Meniscal cartilage injury in the avascular zone is not greatly influenced by alone application of recombinant basic fibroblast growth factor (rbFGF), which may be associated with in vivo absorption and degradation. At recent years, collagen type I has been increasingly applied as a biological carrier.OBJECTIVE: To study the effect of rbFGF/collagen type I composite materials used in repairing meniscal cartilage injuries of rabbits. METHODS: A total of 36 healthy New Zealand rabbits were randomly divided into three groups, including blank control group, rbFGF group and rbFGF/collagen type I group. Models of meniscus injuries in avascular area were established in rabbits. The animals were killed in 2, 6, 12 weeks postoperatively, and meniscus were taken and observed both grossly and microscopically. RESULTS AND CONCLUSION: No significant healing effect was seen in rbFGF group, but proliferation promoting reaction on fibroblasts in synovial membrane at the margin of menisci did exist. The lesion within the avascular portion of the meniscus could be healed by using rbFGF/collagen type I. The reparative tissue was fibrocartilaginous which was different from the normal tissue. Results show a potential effective method of the avascular meniscal injuries repaired by the composite of rbFGF and collagen type I.