| Notch通路与骨髓基质细胞衰老的关系探讨 |
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| 引用本文： | 张可杰,黄丽芳,孙汉英,朱艳,肖毅,黄梅,刘文励. Notch通路与骨髓基质细胞衰老的关系探讨[J]. 中国实验血液学杂志, 2010, 18(2): 410-415 |
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| 作者姓名： | 张可杰 黄丽芳 孙汉英 朱艳 肖毅 黄梅 刘文励 |
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| 作者单位： | 1. 厦门大学附属中山医院血液科,福建医科大学临床教学医院,福建厦门,361004;华中科技大学同济医学院附属同济医院血液科,湖北武汉,430030
2. 华中科技大学同济医学院附属同济医院血液科,湖北武汉,430030 |
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| 基金项目： | 国家自然科学基金资助项目(30570773) |
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| 摘 要： | 本研究探讨Notch通路活化与骨髓基质细胞衰老的关系。用脂质体转染法将Notch1胞内区(ICN)转入体外培养的小鼠骨髓基质细胞,转染后3天,用MTT法检测细胞增殖率;用流式细胞术检测细胞周期分布;用细胞化学法检测衰老相关β-半乳糖苷酶(senescence-associated β galactosidase,SA-β-gal)阳性细胞百分率;用RT-PCR及Western blot法分别检测周期蛋白激酶抑制因子P53、p21Cip1/Waf1基因水平和蛋白水平的表达。结果表明,ICN转染入小鼠骨髓基质细胞后3天,骨髓基质细胞增殖受抑,细胞周期阻滞在G1期,SA-β-gal阳性细胞百分率增加,无论从基因水平还是蛋白水平均显示P53、p21Cip1/Waf1表达增高,与未转染组及转空貭粒组相比差异均具有统计学意义。结论:Notch通路活化可导致骨髓基质细胞衰老,这种作用可能是通过增加p53、p21Cip1/Waf1蛋白的表达实现的。
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| 关 键 词： | Notch通路 骨髓基质细胞 细胞早衰 |
Relation of Notch Pathway to Senescence of Murine Bone Marrow Stromal Cells |
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| ZHANG Ke-Jie,,HUANG Li-Fang,SUN Han-Ying,ZHU Yan,XIAO Yi,HUANG Mei,LIU Wen-Li. Relation of Notch Pathway to Senescence of Murine Bone Marrow Stromal Cells[J]. Journal of experimental hematology, 2010, 18(2): 410-415 |
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| Authors: | ZHANG Ke-Jie HUANG Li-Fang SUN Han-Ying ZHU Yan XIAO Yi HUANG Mei LIU Wen-Li |
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| Affiliation: | ZHANG Ke-Jie1,2,HUANG Li-Fang2,SUN Han-Ying2,ZHU Yan2,XIAO Yi2,HUANG Mei2,LIU Wen-Li2 1Department of Hematology,Zhongshan Hospital,Xiamen University,Fujian Medical University Clinic Teaching Hospital,Xiamen 361004,Fujian Province,China,2Department of Hematology,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,Hubei Province |
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| Abstract: | This study was purposed to investigate the relation of the Notch signaling pathway to senescence of murine bone marrow stromal cells in vitro.Intracellular domain of Notch 1(ICN) was transfected into cultured murine bone marrow stromal cells by lipofectamine transfection.After fransfection for three days the proliferation of transfected cells was measured by MTT,cell cycle distribution was analyzed by flow cytometry.The percentage of senescence associated beta-galactosidase(SA-beta-Gal) positive cells were ... |
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| Keywords: | Notch signaling pathway bone marrow stromal cell premature senescence |
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