补骨脂中补骨脂素的提取及纯化

目的 从中药材补骨脂中提取补骨脂素。方法 补骨脂经粉碎后用50%乙醇浸渍提取,浸提液进行浓缩沉淀,得到膏状物,膏状物用甲醇溶解,活性碳脱杂质,再除去溶剂,放置过夜析出晶体。甲醇洗去稠状物得黄色片状晶体,甲醇重结晶后得针状白色晶体。薄层分析、高效液相色谱分析其纯度。紫外吸收光谱、H1-NMR谱鉴定其结构。结果与结论晶体物得率0.147%,是一个单体,经过波谱分析确定其为补骨脂素。用本方法获取纯品补骨脂素比较简便的方法。

Extraction and purification of psoralen from Psoralea corylifolia L.

Objective To establish a method for extracting psoralen from the seed of Psoralea corylifolia L. Methods Crushed seed of Psoralea corylifolia L. was soaked with 50% ethyl alcohol solution, and the leaching solution was filtrated and distilled to remove alcohol. A slurry of the sediment was obtained and dissolved in methyl alcohol (MeOH), followed by treatment with active carbon for decoloration and evaporation of MeOH, resulting in a lamellar crystal after the sediment was kept still overnight. MeOH was applied to dilute the sticky solution, and after filteration, a yellow crystal was obtained, recrystallization of which in MeOH yielded white needle-like crystals. Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were employed to determine the purity of the resultant substance, and UV and H1-NMR applied to identify its chemical structure. Results and Conclusion The resultant crystal is confirmed to be pure monomers of psoraren with a yield of 0.147% from the seed (m/m) by this simple extraction method.